-
Je něco špatně v tomto záznamu ?
Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM
J. Budna, P. Celichowski, A. Bryja, M. Dyszkiewicz-Konwińska, M. Jeseta, D. Bukowska, P. Antosik, KP. Brüssow, M. Bruska, M. Nowicki, M. Zabel, B. Kempisty,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Free Medical Journals od 2000
Freely Accessible Science Journals od 2000
PubMed Central od 2007
Europe PubMed Central od 2007
ProQuest Central od 2000-03-01
Open Access Digital Library od 2000-01-01
Open Access Digital Library od 2007-01-01
Health & Medicine (ProQuest) od 2000-03-01
ROAD: Directory of Open Access Scholarly Resources od 2000
Odkazy
PubMed
29232894
DOI
10.3390/ijms18122685
Knihovny.cz E-zdroje
- MeSH
- apoptóza MeSH
- buněčná adheze MeSH
- buněčná diferenciace MeSH
- down regulace * MeSH
- genové regulační sítě * MeSH
- IVM techniky veterinární MeSH
- oocyty cytologie metabolismus MeSH
- oogeneze MeSH
- prasata MeSH
- proliferace buněk MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes' maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB⁺ oocytes were directly exposed to microarray assays and RT-qPCR ("before IVM" group), or first in vitro matured and then if classified as BCB⁺ passed to molecular analyses ("after IVM" group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes' successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte's achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.
Department of Anatomy Poznan University of Medical Sciences 60 781 Poznan Poland
Department of Histology and Embryology Poznan University of Medical Sciences 60 781 Poznan Poland
Veterinary Center Nicolaus Copernicus University in Toruń 87 100 Torun Poland
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18033473
- 003
- CZ-PrNML
- 005
- 20181010124918.0
- 007
- ta
- 008
- 181008s2017 sz f 000 00|eng||
- 009
- AR
- 024 7_
- $a 10.3390/ijms18122685 $2 doi
- 035 __
- $a (PubMed)29232894
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Budna, Joanna $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. joanna.budna@wp.pl.
- 245 10
- $a Significant Down-Regulation of "Biological Adhesion" Genes in Porcine Oocytes after IVM / $c J. Budna, P. Celichowski, A. Bryja, M. Dyszkiewicz-Konwińska, M. Jeseta, D. Bukowska, P. Antosik, KP. Brüssow, M. Bruska, M. Nowicki, M. Zabel, B. Kempisty,
- 520 9_
- $a Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes' maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB⁺ oocytes were directly exposed to microarray assays and RT-qPCR ("before IVM" group), or first in vitro matured and then if classified as BCB⁺ passed to molecular analyses ("after IVM" group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes' successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte's achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a apoptóza $7 D017209
- 650 _2
- $a buněčná adheze $7 D002448
- 650 _2
- $a buněčná diferenciace $7 D002454
- 650 _2
- $a proliferace buněk $7 D049109
- 650 12
- $a down regulace $7 D015536
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 12
- $a genové regulační sítě $7 D053263
- 650 _2
- $a IVM techniky $x veterinární $7 D059471
- 650 _2
- $a oocyty $x cytologie $x metabolismus $7 D009865
- 650 _2
- $a oogeneze $7 D009866
- 650 _2
- $a prasata $7 D013552
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Celichowski, Piotr $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. pcelichowski@ump.edu.pl.
- 700 1_
- $a Bryja, Artur $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland. abryja@ump.edu.pl.
- 700 1_
- $a Dyszkiewicz-Konwińska, Marta $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland. mdyszkiewicz@ump.edu.pl. Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, 60-812 Poznan, Poland. mdyszkiewicz@ump.edu.pl.
- 700 1_
- $a Jeseta, Michal $u Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 602 00 Brno, Czech Republic. jeseta@gmail.com.
- 700 1_
- $a Bukowska, Dorota $u Veterinary Center, Nicolaus Copernicus University in Toruń, 87-100 Torun, Poland. lecznicapaw@gmail.com.
- 700 1_
- $a Antosik, Paweł $u Veterinary Center, Nicolaus Copernicus University in Toruń, 87-100 Torun, Poland. antosik67@wp.pl.
- 700 1_
- $a Brüssow, Klaus Peter $u Veterinary Center, Nicolaus Copernicus University in Toruń, 87-100 Torun, Poland. prof.bruessow@gmail.com.
- 700 1_
- $a Bruska, Małgorzata $u Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland. bruska@ump.edu.pl.
- 700 1_
- $a Nowicki, Michał $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. mnowicki@ump.edu.pl.
- 700 1_
- $a Zabel, Maciej $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. mazab@ump.edu.pl. Department of Histology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. mazab@ump.edu.pl.
- 700 1_
- $a Kempisty, Bartosz $u Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland. bkempisty@ump.edu.pl. Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland. bkempisty@ump.edu.pl. Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 602 00 Brno, Czech Republic. bkempisty@ump.edu.pl.
- 773 0_
- $w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 18, č. 12 (2017)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29232894 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20181008 $b ABA008
- 991 __
- $a 20181010125407 $b ABA008
- 999 __
- $a ok $b bmc $g 1340921 $s 1030467
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 18 $c 12 $e 20171211 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
- LZP __
- $a Pubmed-20181008