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Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System
W. Peti, R. Page, E. Boura, B. Różycki,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural
- MeSH
- Endosomal Sorting Complexes Required for Transport chemistry metabolism MeSH
- Protein Conformation MeSH
- Crystallography, X-Ray methods MeSH
- Humans MeSH
- Magnetic Resonance Spectroscopy methods MeSH
- Mitogen-Activated Protein Kinases chemistry metabolism MeSH
- Models, Molecular MeSH
- Scattering, Radiation * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
The integration of complementary molecular methods (including X-ray crystallography, NMR spectroscopy, small angle X-ray/neutron scattering, and computational techniques) is frequently required to obtain a comprehensive understanding of dynamic macromolecular complexes. In particular, these techniques are critical for studying intrinsically disordered protein regions (IDRs) or intrinsically disordered proteins (IDPs) that are part of large protein:protein complexes. Here, we explain how to prepare IDP samples suitable for study using NMR spectroscopy, and describe a novel SAXS modeling method (ensemble refinement of SAXS; EROS) that integrates the results from complementary methods, including crystal structures and NMR chemical shift perturbations, among others, to accurately model SAXS data and describe ensemble structures of dynamic macromolecular complexes.
Department of Chemistry and Biochemistry University of Arizona Tucson AZ 85721 USA
Institute of Physics Polish Academy of Sciences 02668 Warsaw Poland
References provided by Crossref.org
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