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Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism

RCR. Burgos, JC. Schoeman, LJV. Winden, K. Červinková, R. Ramautar, EPA. Van Wijk, M. Cifra, R. Berger, T. Hankemeier, JV. Greef,

. 2017 ; 7 (1) : 1229. [pub] 20170427

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc18033922

In recent years, excessive oxidative metabolism has been reported as a critical determinant of pathogenicity in many diseases. The advent of a simple tool that can provide a physiological readout of oxidative stress would be a major step towards monitoring this dynamic process in biological systems, while also improving our understanding of this process. Ultra-weak photon emission (UPE) has been proposed as a potential tool for measuring oxidative processes due to the association between UPE and reactive oxygen species. Here, we used HL-60 cells as an in vitro model to test the potential of using UPE as readout for dynamically monitoring oxidative stress after inducing respiratory burst. In addition, to probe for possible changes in oxidative metabolism, we performed targeted metabolomics on cell extracts and culture medium. Lastly, we tested the effects of treating cells with the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI). Our results show that UPE can be used as readout for measuring oxidative stress metabolism and related processes.

Citace poskytuje Crossref.org

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$a Burgos, Rosilene Cristina Rossetto $u Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands. r.c.rossetto.burgos@lacdr.leidenuniv.nl. Sino-Dutch Centre for Preventive and Personalised Medicine/Centre for Photonics of Living Systems, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands. r.c.rossetto.burgos@lacdr.leidenuniv.nl.
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$a In recent years, excessive oxidative metabolism has been reported as a critical determinant of pathogenicity in many diseases. The advent of a simple tool that can provide a physiological readout of oxidative stress would be a major step towards monitoring this dynamic process in biological systems, while also improving our understanding of this process. Ultra-weak photon emission (UPE) has been proposed as a potential tool for measuring oxidative processes due to the association between UPE and reactive oxygen species. Here, we used HL-60 cells as an in vitro model to test the potential of using UPE as readout for dynamically monitoring oxidative stress after inducing respiratory burst. In addition, to probe for possible changes in oxidative metabolism, we performed targeted metabolomics on cell extracts and culture medium. Lastly, we tested the effects of treating cells with the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI). Our results show that UPE can be used as readout for measuring oxidative stress metabolism and related processes.
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$a Schoeman, Johannes Cornelius $u Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
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$a Winden, Lennart Jan van $u Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
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$a Červinková, Kateřina $u Institute of Photonics and Electronics, The Czech Academy of Sciences, Chaberská 57, 182 00, Prague, Czech Republic. Faculty of Electrical Engineering, Czech Technical University in Prague, Technická 2, 166 27, Prague, Czech Republic.
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$a Ramautar, Rawi $u Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
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$a Van Wijk, Eduard P A $u Sino-Dutch Centre for Preventive and Personalised Medicine/Centre for Photonics of Living Systems, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
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$a Cifra, Michal $u Institute of Photonics and Electronics, The Czech Academy of Sciences, Chaberská 57, 182 00, Prague, Czech Republic.
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$a Berger, Ruud $u Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
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$a Hankemeier, Thomas $u Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
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$a Greef, Jan van der $u Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands. Sino-Dutch Centre for Preventive and Personalised Medicine/Centre for Photonics of Living Systems, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
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