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Sensitive determination of malondialdehyde in exhaled breath condensate and biological fluids by capillary electrophoresis with laser induced fluorescence detection
J. Lačná, F. Foret, P. Kubáň,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- dechové testy metody MeSH
- elektroforéza kapilární metody MeSH
- fluorescence MeSH
- lasery * MeSH
- lidé MeSH
- malondialdehyd analýza MeSH
- sérum metabolismus MeSH
- sliny metabolismus MeSH
- vydechnutí * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
In this work, a sensitive capillary electrophoresis method with laser induced fluorescence detection for determination of malondialdehyde in various biological fluids was developed. Malondialdehyde reacts with thiobarbituric acid under optimized conditions of pH=2, reaction time of 60min and temperature of 90°C, yielding an adduct that can be separated in a 50mM sodium borate background electrolyte at pH 9. The separation of the formed adduct was accomplished in less than 6min with limit of detection of 1.1nM due to the use of 532nm laser module, exactly matching the maximum excitation wavelength of the formed adduct. The developed method offers unprecedented sensitivity and was for the first time used for analysis of malondialdehyde in exhaled breath condensate. The method proved to be also applicable to other samples of biological fluids, such as blood plasma and saliva.
Department of Bioanalytical Instrumentation CEITEC Masaryk University Veveří 97602 00 Brno Czechia
Department of Chemistry Masaryk University Kotlářská 2 611 37 Brno Czechia
Citace poskytuje Crossref.org
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- $a Lačná, Júlia $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97602 00 Brno, Czechia; Department of Chemistry, Masaryk University, Kotlářská 2, 611 37 Brno, Czechia.
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- $a Sensitive determination of malondialdehyde in exhaled breath condensate and biological fluids by capillary electrophoresis with laser induced fluorescence detection / $c J. Lačná, F. Foret, P. Kubáň,
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- $a In this work, a sensitive capillary electrophoresis method with laser induced fluorescence detection for determination of malondialdehyde in various biological fluids was developed. Malondialdehyde reacts with thiobarbituric acid under optimized conditions of pH=2, reaction time of 60min and temperature of 90°C, yielding an adduct that can be separated in a 50mM sodium borate background electrolyte at pH 9. The separation of the formed adduct was accomplished in less than 6min with limit of detection of 1.1nM due to the use of 532nm laser module, exactly matching the maximum excitation wavelength of the formed adduct. The developed method offers unprecedented sensitivity and was for the first time used for analysis of malondialdehyde in exhaled breath condensate. The method proved to be also applicable to other samples of biological fluids, such as blood plasma and saliva.
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- $a Foret, František $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97602 00 Brno, Czechia; Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveří 97602 00 Brno, Czechia.
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- $a Kubáň, Petr $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97602 00 Brno, Czechia; Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveří 97602 00 Brno, Czechia. Electronic address: petr.kuban@gmail.com.
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