• Je něco špatně v tomto záznamu ?

Domestic Fowl Breed Variation in Egg White Protein Expression: Application of Proteomics and Transcriptomics

B. Bílková, Z. Świderská, L. Zita, D. Laloë, M. Charles, V. Beneš, P. Stopka, M. Vinkler,

. 2018 ; 66 (44) : 11854-11863. [pub] 20181023

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19000330

Avian egg white is essential for protecting and nourishing bird embryos during their development. Being produced in the female magnum, variability in hen oviduct gene expression may affect egg white composition in domestic chickens. Since traditional poultry breeds may represent a source of variation, in the present study we describe the egg white proteome (mass spectrometry) and corresponding magnum transcriptome (high-throughput sequencing) for 20 hens from five domestic fowl breeds (large breeds: Araucana, Czech golden pencilled, Minorca; and small breeds: Booted bantam, Rosecomb bantam). In total, we identified 189 egg white proteins and 16391 magnum-expressed genes. The majority of egg white protein content comprised proteins with an antimicrobial function. Despite general similarity, Between-class Principal Component Analysis revealed significant breed-specific variability in protein abundances, differentiating especially small and large breeds. Though we found strong association between magnum mRNA expression and egg white protein abundance across genes, coinertia analysis revealed no transcriptome/proteome costructure at the individual level. Our study is the first to show variation in protein abundances in egg white across chicken breeds with potential effects on egg quality, biosafety, and chick development. The observed interindividual variation probably results from post-transcriptional regulation creating a discrepancy between proteomic and transcriptomic data.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19000330
003      
CZ-PrNML
005      
20190122115825.0
007      
ta
008      
190107s2018 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1021/acs.jafc.8b03099 $2 doi
035    __
$a (PubMed)30296079
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Bílková, Barbora $u Charles University , Faculty of Science, Department of Zoology , Prague , Czech Republic.
245    10
$a Domestic Fowl Breed Variation in Egg White Protein Expression: Application of Proteomics and Transcriptomics / $c B. Bílková, Z. Świderská, L. Zita, D. Laloë, M. Charles, V. Beneš, P. Stopka, M. Vinkler,
520    9_
$a Avian egg white is essential for protecting and nourishing bird embryos during their development. Being produced in the female magnum, variability in hen oviduct gene expression may affect egg white composition in domestic chickens. Since traditional poultry breeds may represent a source of variation, in the present study we describe the egg white proteome (mass spectrometry) and corresponding magnum transcriptome (high-throughput sequencing) for 20 hens from five domestic fowl breeds (large breeds: Araucana, Czech golden pencilled, Minorca; and small breeds: Booted bantam, Rosecomb bantam). In total, we identified 189 egg white proteins and 16391 magnum-expressed genes. The majority of egg white protein content comprised proteins with an antimicrobial function. Despite general similarity, Between-class Principal Component Analysis revealed significant breed-specific variability in protein abundances, differentiating especially small and large breeds. Though we found strong association between magnum mRNA expression and egg white protein abundance across genes, coinertia analysis revealed no transcriptome/proteome costructure at the individual level. Our study is the first to show variation in protein abundances in egg white across chicken breeds with potential effects on egg quality, biosafety, and chick development. The observed interindividual variation probably results from post-transcriptional regulation creating a discrepancy between proteomic and transcriptomic data.
650    _2
$a zvířata $7 D000818
650    _2
$a hospodářská zvířata $x klasifikace $x genetika $x metabolismus $7 D000829
650    _2
$a kur domácí $x klasifikace $x genetika $x metabolismus $7 D002645
650    _2
$a vaječné proteiny $x chemie $x genetika $x metabolismus $7 D004527
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a stanovení celkové genové exprese $7 D020869
650    _2
$a proteom $x chemie $x genetika $x metabolismus $7 D020543
650    _2
$a proteomika $7 D040901
655    _2
$a časopisecké články $7 D016428
700    1_
$a Świderská, Zuzana $u Charles University , Faculty of Science, Department of Zoology , Prague , Czech Republic. Charles University , Faculty of Science, Department of Cell Biology , Prague , Czech Republic.
700    1_
$a Zita, Lukáš $u Czech University of Life Sciences , Faculty of Agrobiology, Food and Natural Resources, Department of Animal Science , Prague , Czech Republic.
700    1_
$a Laloë, Denis $u GABI, INRA, AgroParisTech , Université Paris-Saclay , Jouy-en-Josas , France.
700    1_
$a Charles, Mathieu $u GABI, INRA, AgroParisTech , Université Paris-Saclay , Jouy-en-Josas , France.
700    1_
$a Beneš, Vladimír $u European Molecular Biology Laboratory , Heidelberg 69117 , Germany.
700    1_
$a Stopka, Pavel $u Charles University , Faculty of Science, Department of Zoology , Prague , Czech Republic.
700    1_
$a Vinkler, Michal $u Charles University , Faculty of Science, Department of Zoology , Prague , Czech Republic.
773    0_
$w MED00002503 $t Journal of agricultural and food chemistry $x 1520-5118 $g Roč. 66, č. 44 (2018), s. 11854-11863
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30296079 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190122120045 $b ABA008
999    __
$a ok $b bmc $g 1363816 $s 1038453
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 66 $c 44 $d 11854-11863 $e 20181023 $i 1520-5118 $m Journal of agricultural and food chemistry $n J Agric Food Chem $x MED00002503
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

    Možnosti archivace