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Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator
V. Pitschmann, L. Matějovský, M. Lobotka, J. Dědič, M. Urban, M. Dymák,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
NLK
Directory of Open Access Journals
od 2011
Free Medical Journals
od 2011
PubMed Central
od 2011
Europe PubMed Central
od 2011
ProQuest Central
od 2011-03-01
Open Access Digital Library
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Health & Medicine (ProQuest)
od 2011-03-01
ROAD: Directory of Open Access Scholarly Resources
od 2011
PubMed
30181477
DOI
10.3390/bios8030081
Knihovny.cz E-zdroje
- MeSH
- biosenzitivní techniky metody MeSH
- chemické bojové látky analýza MeSH
- cholinesterasové inhibitory analýza MeSH
- kolorimetrie metody MeSH
- lissaminová zelená barviva chemie MeSH
- Publikační typ
- časopisecké články MeSH
Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable, and very simple detection of agents in air, in water, and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualized by using Ellman's reagent as a chromogenic indicator. The modification basically consists of a much more distinct color response of the biosensor, attained through optimization of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin, and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 s, inhibition efficiency 25%).
Citace poskytuje Crossref.org
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