Metallothionein (MT) plays the important role in the detoxification of heavy metals, protection against oxidative compounds and as a prognostic marker in the development of tumors. It is important to find selective, stable and sensitive tools and probes to evaluate the presence of MT in biological fluids or tissues. QDs linked with ligands such as peptides or small molecules are a promising tool for selective, fast, and sensitive tagging and imaging in medicine. In previous findings, the authors proved the possibility of interaction with QDs (particularly with CdTe) and analyzed the stability of the formed complexes between CdTe and MT during incubation over time. Following that, an initial analysis of the interactions between CdTe quantum dots (QDs) and human metallothionein (MT) was performed. Complexes of mercaptosuccinic acid-covered CdTe QDs + MT were investigated using fluorescence intensity changes along a timeline, quenching analysis, stability interpretation based on zeta potential, and quenching intensity. Based on the preliminary results, it appears as though the possible interactions depend on the size of the CdTe QDs. Additionally, the formation of complexes between CdTe and human MT likely depends mostly on structural changes and conformational reorganization rather than on electrostatic interactions. Both types of interactions are responsible for complex creation and stabilization.

Central European Institute of Technology Brno University of Technology Purkynova 656 123 CZ 61200 Brno Czech Republic

Department of Biomedical and Environmental Analyses Faculty of Pharmacy with Division of Laboratory Diagnostic Wroclaw Medical University Borowska 211 PL 50 556 Wroclaw Poland

Department of Chemistry and Biochemistry Faculty of AgriSciences Mendel University in Brno Zemedelska 1 1665 CZ 61300 Brno Czech Republic

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