-
Something wrong with this record ?
Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes
K. Souček, A. Malenovská, Z. Kahounová, J. Remšík, Z. Holubcová, T. Soukup, D. Kurfürstová, J. Bouchal, T. Suchánková, E. Slabáková, A. Hampl,
Language English Country Netherlands
Document type Journal Article
Grant support
NV15-33999A
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
Free Medical Journals
from 2008 to 1 year ago
PubMed Central
from 1997 to 1 year ago
Europe PubMed Central
from 1997 to 1 year ago
ProQuest Central
from 1999-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2011-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1999-01-01 to 1 year ago
Public Health Database (ProQuest)
from 1999-01-01 to 1 year ago
- MeSH
- Cell Differentiation genetics MeSH
- Adult MeSH
- Fertilization in Vitro MeSH
- Granulosa Cells metabolism MeSH
- Follicular Fluid metabolism MeSH
- Humans MeSH
- Oocyte Retrieval MeSH
- Oocytes metabolism MeSH
- Growth Differentiation Factor 15 genetics isolation & purification MeSH
- Gene Expression Regulation, Developmental MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
PURPOSE: The purpose of the study was to determine whether the GDF-15 is present in follicular fluid; to evaluate if there is a relation between follicular and serum levels of GDF-15 and fertility status of study subjects; and to test whether granulosa cells, oocytes, or both produce GDF-15. METHODS: This study used follicular fluid (FF, serum, and oocytes obtained under informed consent from women undergoing oocyte retrieval for in vitro fertilization. It also used ovaries from deceased preterm newborns. Collection of FF and blood at the time of oocyte retrieval, ELISA and western blot were performed to determine levels and forms of GDF-15. Concentrations of GDF-15 in FF and serum, its expression in ovarian tissue, and secretion from granulosa cells were analyzed. RESULTS: GDF-15 concentration in FF ranged from 35 to 572 ng/ml, as determined by ELISA. Western blot analysis revealed the GDF-15 pro-dimer only in FF. Both normal healthy and cancerous granulosa cells secreted GDF-15 into culture media. Primary oocytes displayed cytoplasmic GDF-15 positivity in immunostained newborn ovaries, and its expression was also observed in fully grown human oocytes. CONCLUSIONS: To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19000613
- 003
- CZ-PrNML
- 005
- 20220216131914.0
- 007
- ta
- 008
- 190107s2018 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s10815-018-1230-5 $2 doi
- 035 __
- $a (PubMed)29948426
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Souček, Karel $u Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65, Brno, Czech Republic. ksoucek@ibp.cz. International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic. ksoucek@ibp.cz.
- 245 10
- $a Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes / $c K. Souček, A. Malenovská, Z. Kahounová, J. Remšík, Z. Holubcová, T. Soukup, D. Kurfürstová, J. Bouchal, T. Suchánková, E. Slabáková, A. Hampl,
- 520 9_
- $a PURPOSE: The purpose of the study was to determine whether the GDF-15 is present in follicular fluid; to evaluate if there is a relation between follicular and serum levels of GDF-15 and fertility status of study subjects; and to test whether granulosa cells, oocytes, or both produce GDF-15. METHODS: This study used follicular fluid (FF, serum, and oocytes obtained under informed consent from women undergoing oocyte retrieval for in vitro fertilization. It also used ovaries from deceased preterm newborns. Collection of FF and blood at the time of oocyte retrieval, ELISA and western blot were performed to determine levels and forms of GDF-15. Concentrations of GDF-15 in FF and serum, its expression in ovarian tissue, and secretion from granulosa cells were analyzed. RESULTS: GDF-15 concentration in FF ranged from 35 to 572 ng/ml, as determined by ELISA. Western blot analysis revealed the GDF-15 pro-dimer only in FF. Both normal healthy and cancerous granulosa cells secreted GDF-15 into culture media. Primary oocytes displayed cytoplasmic GDF-15 positivity in immunostained newborn ovaries, and its expression was also observed in fully grown human oocytes. CONCLUSIONS: To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a buněčná diferenciace $x genetika $7 D002454
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a fertilizace in vitro $7 D005307
- 650 _2
- $a folikulární tekutina $x metabolismus $7 D015571
- 650 _2
- $a vývojová regulace genové exprese $7 D018507
- 650 _2
- $a folikulární buňky $x metabolismus $7 D006107
- 650 _2
- $a růstový diferenciační faktor 15 $x genetika $x izolace a purifikace $7 D055436
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a odběr oocytu $7 D054315
- 650 _2
- $a oocyty $x metabolismus $7 D009865
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Malenovská, Alice $u Reprofit International Clinic of Reproductive Medicine, Brno, Czech Republic.
- 700 1_
- $a Kahounová, Zuzana $u Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65, Brno, Czech Republic. International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
- 700 1_
- $a Remšík, Ján $u Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65, Brno, Czech Republic. International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic. Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
- 700 1_
- $a Holubcová, Zuzana $u Reprofit International Clinic of Reproductive Medicine, Brno, Czech Republic. Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 3, 625 00, Brno, Czech Republic.
- 700 1_
- $a Soukup, Tomáš $u Faculty of Medicine in Hradec Králové, Department of Histology and Embryology, Charles University in Prague, Hradec Králové, Czech Republic.
- 700 1_
- $a Kurfürstová, Daniela $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic. $7 xx0269558
- 700 1_
- $a Bouchal, Jan $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
- 700 1_
- $a Suchánková, Tereza $u Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65, Brno, Czech Republic.
- 700 1_
- $a Slabáková, Eva $u Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65, Brno, Czech Republic.
- 700 1_
- $a Hampl, Aleš $u International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic. ahampl@med.muni.cz. Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 3, 625 00, Brno, Czech Republic. ahampl@med.muni.cz.
- 773 0_
- $w MED00002533 $t Journal of assisted reproduction and genetics $x 1573-7330 $g Roč. 35, č. 8 (2018), s. 1407-1417
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29948426 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190107 $b ABA008
- 991 __
- $a 20220216131909 $b ABA008
- 999 __
- $a ok $b bmc $g 1364662 $s 1038736
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 35 $c 8 $d 1407-1417 $e 20180613 $i 1573-7330 $m Journal of assisted reproduction and genetics $n J Assist Reprod Genet $x MED00002533
- GRA __
- $a NV15-33999A $p MZ0
- LZP __
- $a Pubmed-20190107
