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Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes
K. Souček, A. Malenovská, Z. Kahounová, J. Remšík, Z. Holubcová, T. Soukup, D. Kurfürstová, J. Bouchal, T. Suchánková, E. Slabáková, A. Hampl,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
Grantová podpora
NV15-33999A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Zdroj
NLK
Free Medical Journals
od 2008 do Před 1 rokem
PubMed Central
od 1997 do Před 1 rokem
Europe PubMed Central
od 1997 do Před 1 rokem
ProQuest Central
od 1999-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2011-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1999-01-01 do Před 1 rokem
Public Health Database (ProQuest)
od 1999-01-01 do Před 1 rokem
- MeSH
- buněčná diferenciace genetika MeSH
- dospělí MeSH
- fertilizace in vitro MeSH
- folikulární buňky metabolismus MeSH
- folikulární tekutina metabolismus MeSH
- lidé MeSH
- odběr oocytu MeSH
- oocyty metabolismus MeSH
- růstový diferenciační faktor 15 genetika izolace a purifikace MeSH
- vývojová regulace genové exprese MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
PURPOSE: The purpose of the study was to determine whether the GDF-15 is present in follicular fluid; to evaluate if there is a relation between follicular and serum levels of GDF-15 and fertility status of study subjects; and to test whether granulosa cells, oocytes, or both produce GDF-15. METHODS: This study used follicular fluid (FF, serum, and oocytes obtained under informed consent from women undergoing oocyte retrieval for in vitro fertilization. It also used ovaries from deceased preterm newborns. Collection of FF and blood at the time of oocyte retrieval, ELISA and western blot were performed to determine levels and forms of GDF-15. Concentrations of GDF-15 in FF and serum, its expression in ovarian tissue, and secretion from granulosa cells were analyzed. RESULTS: GDF-15 concentration in FF ranged from 35 to 572 ng/ml, as determined by ELISA. Western blot analysis revealed the GDF-15 pro-dimer only in FF. Both normal healthy and cancerous granulosa cells secreted GDF-15 into culture media. Primary oocytes displayed cytoplasmic GDF-15 positivity in immunostained newborn ovaries, and its expression was also observed in fully grown human oocytes. CONCLUSIONS: To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes.
Citace poskytuje Crossref.org
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- $a Souček, Karel $u Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65, Brno, Czech Republic. ksoucek@ibp.cz. International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic. ksoucek@ibp.cz.
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- $a Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes / $c K. Souček, A. Malenovská, Z. Kahounová, J. Remšík, Z. Holubcová, T. Soukup, D. Kurfürstová, J. Bouchal, T. Suchánková, E. Slabáková, A. Hampl,
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- $a PURPOSE: The purpose of the study was to determine whether the GDF-15 is present in follicular fluid; to evaluate if there is a relation between follicular and serum levels of GDF-15 and fertility status of study subjects; and to test whether granulosa cells, oocytes, or both produce GDF-15. METHODS: This study used follicular fluid (FF, serum, and oocytes obtained under informed consent from women undergoing oocyte retrieval for in vitro fertilization. It also used ovaries from deceased preterm newborns. Collection of FF and blood at the time of oocyte retrieval, ELISA and western blot were performed to determine levels and forms of GDF-15. Concentrations of GDF-15 in FF and serum, its expression in ovarian tissue, and secretion from granulosa cells were analyzed. RESULTS: GDF-15 concentration in FF ranged from 35 to 572 ng/ml, as determined by ELISA. Western blot analysis revealed the GDF-15 pro-dimer only in FF. Both normal healthy and cancerous granulosa cells secreted GDF-15 into culture media. Primary oocytes displayed cytoplasmic GDF-15 positivity in immunostained newborn ovaries, and its expression was also observed in fully grown human oocytes. CONCLUSIONS: To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes.
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- $a Kahounová, Zuzana $u Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65, Brno, Czech Republic. International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
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- $a Remšík, Ján $u Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65, Brno, Czech Republic. International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic. Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
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- $a Hampl, Aleš $u International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic. ahampl@med.muni.cz. Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 3, 625 00, Brno, Czech Republic. ahampl@med.muni.cz.
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