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Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon

I. Lebeda, C. Steinbach, M. Flajšhans,

. 2018 ; 92 (6) : 1819-1831. [pub] 20180423

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu hodnotící studie, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19000849

The efficacy of ploidy analysis for separating progeny of Siberian sturgeon Acipenser baerii after induced gynogenesis was demonstrated using sperm of a paternal species differing in ploidy level from the maternal species. Gynogenesis was induced in tetraploid A. baerii with UV-C irradiated sperm from the diploid sterlet Acipenser ruthenus and vice-versa. The success of sperm UV irradiation and diploidy restoration by heat-shock was estimated based on the ploidy level of progeny, confirmed by microsatellite parentage assignment. Hatching rates of interspecific gynogenotes were comparable with rates reported for gynogenesis induction using sperm and eggs of the same species. Juvenile mortality was similar to that observed in the control hybrids. The efficiency and reliability of this method may foster its use for production of gynogenotes in aquaculture, potentially allowing interspecific gynogenesis to replace intraspecific.

Citace poskytuje Crossref.org

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