-
Je něco špatně v tomto záznamu ?
Antiplasmodial activity of tick defensins in a mouse model of malaria
J. Couto, M. Tonk, J. Ferrolho, S. Antunes, A. Vilcinskas, J. de la Fuente, A. Domingos, A. Cabezas-Cruz,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antimalarika aplikace a dávkování škodlivé účinky terapeutické užití MeSH
- defensiny aplikace a dávkování škodlivé účinky terapeutické užití MeSH
- intravenózní podání MeSH
- klíště chemie MeSH
- malárie farmakoterapie parazitologie MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- parazitemie farmakoterapie parazitologie MeSH
- Plasmodium účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Malaria is a mosquito-borne disease affecting millions of people mainly in Sub-Saharan Africa, Asia and some South American countries. Drug resistance to first-line antimalarial drugs (e.g. chloroquine, sulfadoxine-pyrimethamine and artemisinin) is a major constrain in malaria control. Antimicrobial peptides (AMPs) have shown promising results in controlling Plasmodium spp. parasitemia in in vitro and in vivo models of infection. Defensins are AMPs that act primarily by disrupting the integrity of cell membranes of invasive microbes. We previously showed that defensins from the tick Ixodes ricinus inhibited significantly the growth of P. falciparum in vitro, a property that was conserved during evolution. Here, we tested the activity of three I. ricinus defensins against P. chabaudi in mice. A single dose of defensin (120 μl of 1 mg/ml solution) was administered intravenously to P. chabaudi-infected mice, and the parasitemia was followed for 24 h post-treatment. Defensin treatment inhibited significantly the replication (measured as increases in parasitemia) of P. chabaudi after 1 h and 12 h of treatment. Furthermore, defensin injection was not associated with toxicity. These results agreed with the previous report of antiplasmodial activity of tick defensins against P. falciparum in vitro and justify further studies for the use of tick defensins to control malaria.
Faculty of Science University of South Bohemia 37005 České Budějovice Czech Republic
SaBio Instituto de Investigación en Recursos Cinegéticos IREC 13005 Ciudad Real Spain
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19000855
- 003
- CZ-PrNML
- 005
- 20190121105417.0
- 007
- ta
- 008
- 190107s2018 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.ttbdis.2018.03.011 $2 doi
- 035 __
- $a (PubMed)29567145
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Couto, Joana $u Global Health and Tropical Medicine - Instituto de Higiene e Medicina, Universidade Nova de Lisboa (GHMT-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
- 245 10
- $a Antiplasmodial activity of tick defensins in a mouse model of malaria / $c J. Couto, M. Tonk, J. Ferrolho, S. Antunes, A. Vilcinskas, J. de la Fuente, A. Domingos, A. Cabezas-Cruz,
- 520 9_
- $a Malaria is a mosquito-borne disease affecting millions of people mainly in Sub-Saharan Africa, Asia and some South American countries. Drug resistance to first-line antimalarial drugs (e.g. chloroquine, sulfadoxine-pyrimethamine and artemisinin) is a major constrain in malaria control. Antimicrobial peptides (AMPs) have shown promising results in controlling Plasmodium spp. parasitemia in in vitro and in vivo models of infection. Defensins are AMPs that act primarily by disrupting the integrity of cell membranes of invasive microbes. We previously showed that defensins from the tick Ixodes ricinus inhibited significantly the growth of P. falciparum in vitro, a property that was conserved during evolution. Here, we tested the activity of three I. ricinus defensins against P. chabaudi in mice. A single dose of defensin (120 μl of 1 mg/ml solution) was administered intravenously to P. chabaudi-infected mice, and the parasitemia was followed for 24 h post-treatment. Defensin treatment inhibited significantly the replication (measured as increases in parasitemia) of P. chabaudi after 1 h and 12 h of treatment. Furthermore, defensin injection was not associated with toxicity. These results agreed with the previous report of antiplasmodial activity of tick defensins against P. falciparum in vitro and justify further studies for the use of tick defensins to control malaria.
- 650 _2
- $a intravenózní podání $7 D061605
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a antimalarika $x aplikace a dávkování $x škodlivé účinky $x terapeutické užití $7 D000962
- 650 _2
- $a defensiny $x aplikace a dávkování $x škodlivé účinky $x terapeutické užití $7 D023082
- 650 _2
- $a modely nemocí na zvířatech $7 D004195
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a klíště $x chemie $7 D018884
- 650 _2
- $a malárie $x farmakoterapie $x parazitologie $7 D008288
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši inbrední BALB C $7 D008807
- 650 _2
- $a parazitemie $x farmakoterapie $x parazitologie $7 D018512
- 650 _2
- $a Plasmodium $x účinky léků $7 D010961
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Tonk, Miray $u Fraunhofer Institute for Molecular Biology and Applied Ecology, Winchester Strasse 2, D-35394 Giessen, Germany.
- 700 1_
- $a Ferrolho, Joana $u Global Health and Tropical Medicine - Instituto de Higiene e Medicina, Universidade Nova de Lisboa (GHMT-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
- 700 1_
- $a Antunes, Sandra $u Global Health and Tropical Medicine - Instituto de Higiene e Medicina, Universidade Nova de Lisboa (GHMT-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
- 700 1_
- $a Vilcinskas, Andreas $u Fraunhofer Institute for Molecular Biology and Applied Ecology, Winchester Strasse 2, D-35394 Giessen, Germany; Institute for Insect Biotechnology, Justus Liebig University of Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
- 700 1_
- $a de la Fuente, José $u SaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), 13005 Ciudad Real, Spain; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 USA.
- 700 1_
- $a Domingos, Ana $u Global Health and Tropical Medicine - Instituto de Higiene e Medicina, Universidade Nova de Lisboa (GHMT-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal. Electronic address: adomingos@ihmt.unl.pt.
- 700 1_
- $a Cabezas-Cruz, Alejandro $u UMR BIPAR, INRA, ANSES, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, 94700, France; Faculty of Science, University of South Bohemia, 37005 České Budějovice, Czech Republic; Institute of Parasitology, Biology Center, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic. Electronic address: cabezasalejandrocruz@gmail.com.
- 773 0_
- $w MED00167597 $t Ticks and tick-borne diseases $x 1877-9603 $g Roč. 9, č. 4 (2018), s. 844-849
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29567145 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190107 $b ABA008
- 991 __
- $a 20190121105635 $b ABA008
- 999 __
- $a ok $b bmc $g 1364847 $s 1038978
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 9 $c 4 $d 844-849 $e 20180315 $i 1877-9603 $m Ticks and tick-borne diseases $n Ticks Tick Borne Dis $x MED00167597
- LZP __
- $a Pubmed-20190107
