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Alimentary Tract Transcriptome Analysis of the Tea Geometrid, Ectropis oblique (Lepidoptera: Geometridae)
J. Wang, G. Lin, K. Batool, S. Zhang, M. Chen, J. Xu, J. Wu, L. Jin, I. Gelbic, L. Xu, L. Zhang, X. Guan,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2014-02-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2014-02-01 do Před 1 rokem
PubMed
29546335
DOI
10.1093/jee/toy010
Knihovny.cz E-zdroje
- MeSH
- larva genetika růst a vývoj MeSH
- můry genetika růst a vývoj MeSH
- sekvenční analýza DNA MeSH
- stanovení celkové genové exprese MeSH
- transkriptom * MeSH
- trávicí systém MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Ectropis oblique Prout (Lepidoptera: Geometridae) is one of the main pests that damages the tea crop in Southeast Asia. To understand the molecular mechanisms of its feeding biology, transcriptomes of the alimentary tract (AT) and of the body minus the AT of E. oblique were successfully sequenced and analyzed in this study. A total of 36,950 unigenes from de novo sequences were assembled. After analysis using six annotation databases (e.g., Gene Ontology, Kyoto Encyclopedia of Genes and Genome, and NCBI nr), a series of putative genes were found for this insect species that were related to digestion, detoxification, the immune system, and Bacillus thuringiensis (Bt) receptors. From this series of genes, 21 were randomly selected to verify the relative expression levels of transcripts using quantitative real-time polymerase chain reaction. These results will provide an invaluable genomic resource for future studies on the molecular mechanisms of E. oblique, which will be useful in developing biological control strategies for this pest.
Citace poskytuje Crossref.org
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