Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

The Optimal Timing for Pancreatic Islet Transplantation into Subcutaneous Scaffolds Assessed by Multimodal Imaging

A. Gálisová, E. Fábryová, E. Sticová, L. Kosinová, M. Jirátová, V. Herynek, Z. Berková, J. Kříž, M. Hájek, D. Jirák,

. 2017 ; 2017 (-) : 5418495. [pub] 20171226

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19000920

Grant support
NV16-28254A MZ0 CEP Register
NV16-28254A MZ0 CEP Register

Subcutaneously implanted polymeric scaffolds represent an alternative transplantation site for pancreatic islets (PIs) with the option of vascularisation enhancement by mesenchymal stem cells (MSC). Nevertheless, a proper timing of the transplantation steps is crucial. In this study, scaffolds supplemented with plastic rods were implanted into diabetic rats and two timing schemes for subsequent transplantation of bioluminescent PIs (4 or 7 days after rod removal) were examined by multimodal imaging. The cavities were left to heal spontaneously or with 10 million injected MSCs. Morphological and vascularisation changes were examined by MRI, while the localisation and viability of transplanted islets were monitored by bioluminescence imaging. The results show that PIs transplanted 4 days after rod removal showed the higher optical signal and vascularisation compared to transplantation after 7 days. MSCs slightly improved vascularisation of the graft but hindered therapeutic efficiency of PIs. Long-term glycaemia normalisation (4 months) was attained in 80% of animals. In summary, multimodal imaging confirmed the long-term survival and function of transplanted PIs in the devices. The best outcome was reached with PIs transplanted on day 4 after rod removal and therefore the suggested protocol holds a potential for further applications.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19000920
003      
CZ-PrNML
005      
20200121141306.0
007      
ta
008      
190107s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1155/2017/5418495 $2 doi
035    __
$a (PubMed)29440984
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Gálisová, Andrea $u MR Unit, Department of Radiodiagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
245    14
$a The Optimal Timing for Pancreatic Islet Transplantation into Subcutaneous Scaffolds Assessed by Multimodal Imaging / $c A. Gálisová, E. Fábryová, E. Sticová, L. Kosinová, M. Jirátová, V. Herynek, Z. Berková, J. Kříž, M. Hájek, D. Jirák,
520    9_
$a Subcutaneously implanted polymeric scaffolds represent an alternative transplantation site for pancreatic islets (PIs) with the option of vascularisation enhancement by mesenchymal stem cells (MSC). Nevertheless, a proper timing of the transplantation steps is crucial. In this study, scaffolds supplemented with plastic rods were implanted into diabetic rats and two timing schemes for subsequent transplantation of bioluminescent PIs (4 or 7 days after rod removal) were examined by multimodal imaging. The cavities were left to heal spontaneously or with 10 million injected MSCs. Morphological and vascularisation changes were examined by MRI, while the localisation and viability of transplanted islets were monitored by bioluminescence imaging. The results show that PIs transplanted 4 days after rod removal showed the higher optical signal and vascularisation compared to transplantation after 7 days. MSCs slightly improved vascularisation of the graft but hindered therapeutic efficiency of PIs. Long-term glycaemia normalisation (4 months) was attained in 80% of animals. In summary, multimodal imaging confirmed the long-term survival and function of transplanted PIs in the devices. The best outcome was reached with PIs transplanted on day 4 after rod removal and therefore the suggested protocol holds a potential for further applications.
650    _2
$a alografty $7 D064591
650    _2
$a zvířata $7 D000818
650    12
$a experimentální diabetes mellitus $x diagnostické zobrazování $x chirurgie $7 D003921
650    12
$a přežívání štěpu $7 D006085
650    12
$a Langerhansovy ostrůvky $x krevní zásobení $x diagnostické zobrazování $x metabolismus $x patologie $7 D007515
650    _2
$a transplantace Langerhansových ostrůvků $x metody $7 D016381
650    12
$a luminiscenční měření $7 D008163
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a transplantace mezenchymálních kmenových buněk $7 D045164
650    _2
$a mezenchymální kmenové buňky $x metabolismus $x patologie $7 D059630
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani transgenní $7 D055647
650    12
$a tkáňové podpůrné struktury $7 D054457
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Fábryová, Eva $u Centre of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Sticová, Eva $u Department of Clinical and Transplant Pathology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Department of Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
700    1_
$a Kosinová, Lucie $u Centre of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Jirátová, Markéta $u MR Unit, Department of Radiodiagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Herynek, Vít $u MR Unit, Department of Radiodiagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Berková, Zuzana $u Centre of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Kříž, Jan $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Hájek, Milan $u MR Unit, Department of Radiodiagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
700    1_
$a Jirák, Daniel $u MR Unit, Department of Radiodiagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
773    0_
$w MED00180205 $t Contrast media & molecular imaging $x 1555-4317 $g Roč. 2017, č. - (2017), s. 5418495
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29440984 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20200121141643 $b ABA008
999    __
$a ok $b bmc $g 1364893 $s 1039043
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 2017 $c - $d 5418495 $e 20171226 $i 1555-4317 $m Contrast Media Mol Imaging $n Contrast Media Mol Imaging $x MED00180205
GRA    __
$a NV16-28249A $a NV16-28254A $p MZ0 $p MZ0
GRA    __
$a NV16-28249A $a NV16-28254A $p MZ0 $p MZ0
LZP    __
$a Pubmed-20190107

Find record

Citation metrics

Archiving options