Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity

BM. Kuhn, T. Nodzyński, S. Errafi, R. Bucher, S. Gupta, B. Aryal, P. Dobrev, L. Bigler, M. Geisler, E. Zažímalová, J. Friml, C. Ringli,

. 2017 ; 7 (-) : 41906. [pub] 20170206

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19001322

The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Flavonols have been shown to alter auxin transport activity and changes in flavonol accumulation in the Arabidopsis thaliana rol1-2 mutant cause defects in auxin transport and seedling development. A new mutation in ROOTS CURL IN NPA 1 (RCN1), encoding a regulatory subunit of the phosphatase PP2A, was found to suppress the growth defects of rol1-2 without changing the flavonol content. rol1-2 rcn1-3 double mutants show wild type-like auxin transport activity while levels of free auxin are not affected by rcn1-3. In the rol1-2 mutant, PIN2 shows a flavonol-induced basal-to-apical shift in polar localization which is reversed in the rol1-2 rcn1-3 to basal localization. In vivo analysis of PINOID action, a kinase known to influence PIN protein localization in a PP2A-antagonistic manner, revealed a negative impact of flavonols on PINOID activity. Together, these data suggest that flavonols affect auxin transport by modifying the antagonistic kinase/phosphatase equilibrium.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19001322
003      
CZ-PrNML
005      
20190108130320.0
007      
ta
008      
190107s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/srep41906 $2 doi
035    __
$a (PubMed)28165500
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Kuhn, Benjamin M $u Institute of Plant and Microbial Biology, University of Zurich, Zurich Switzerland.
245    10
$a Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity / $c BM. Kuhn, T. Nodzyński, S. Errafi, R. Bucher, S. Gupta, B. Aryal, P. Dobrev, L. Bigler, M. Geisler, E. Zažímalová, J. Friml, C. Ringli,
520    9_
$a The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Flavonols have been shown to alter auxin transport activity and changes in flavonol accumulation in the Arabidopsis thaliana rol1-2 mutant cause defects in auxin transport and seedling development. A new mutation in ROOTS CURL IN NPA 1 (RCN1), encoding a regulatory subunit of the phosphatase PP2A, was found to suppress the growth defects of rol1-2 without changing the flavonol content. rol1-2 rcn1-3 double mutants show wild type-like auxin transport activity while levels of free auxin are not affected by rcn1-3. In the rol1-2 mutant, PIN2 shows a flavonol-induced basal-to-apical shift in polar localization which is reversed in the rol1-2 rcn1-3 to basal localization. In vivo analysis of PINOID action, a kinase known to influence PIN protein localization in a PP2A-antagonistic manner, revealed a negative impact of flavonols on PINOID activity. Together, these data suggest that flavonols affect auxin transport by modifying the antagonistic kinase/phosphatase equilibrium.
650    _2
$a Arabidopsis $x účinky léků $x genetika $x metabolismus $7 D017360
650    _2
$a proteiny huseníčku $x genetika $x metabolismus $7 D029681
650    _2
$a flavonoidy $x farmakologie $7 D005419
650    _2
$a glukosyltransferasy $x genetika $x metabolismus $7 D005964
650    _2
$a kyseliny indoloctové $x metabolismus $7 D007210
650    _2
$a mutace $7 D009154
650    _2
$a proteinfosfatasa 2 $x genetika $x metabolismus $7 D054648
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Nodzyński, Tomasz $u Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic.
700    1_
$a Errafi, Sanae $u Institute of Plant and Microbial Biology, University of Zurich, Zurich Switzerland.
700    1_
$a Bucher, Rahel $u Institute of Chemistry, University of Zurich, Zurich, Switzerland.
700    1_
$a Gupta, Shibu $u Institute of Plant and Microbial Biology, University of Zurich, Zurich Switzerland.
700    1_
$a Aryal, Bibek $u Department of Biology - geislerLab, University of Fribourg, Fribourg, Switzerland.
700    1_
$a Dobrev, Petre $u Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Bigler, Laurent $u Institute of Chemistry, University of Zurich, Zurich, Switzerland.
700    1_
$a Geisler, Markus $u Department of Biology - geislerLab, University of Fribourg, Fribourg, Switzerland.
700    1_
$a Zažímalová, Eva $u Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Friml, Jiří $u Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
700    1_
$a Ringli, Christoph $u Institute of Plant and Microbial Biology, University of Zurich, Zurich Switzerland.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 7, č. - (2017), s. 41906
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28165500 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190108130521 $b ABA008
999    __
$a ok $b bmc $g 1365206 $s 1039445
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 7 $c - $d 41906 $e 20170206 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

Možnosti archivace