• Je něco špatně v tomto záznamu ?

Role of Cnot6l in maternal mRNA turnover

F. Horvat, H. Fulka, R. Jankele, R. Malik, M. Jun, K. Solcova, R. Sedlacek, K. Vlahovicek, RM. Schultz, P. Svoboda,

. 2018 ; 1 (4) : e201800084. [pub] 20180716

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19001529

Removal of poly(A) tail is an important mechanism controlling eukaryotic mRNA turnover. The major eukaryotic deadenylase complex CCR4-NOT contains two deadenylase components, CCR4 and CAF1, for which mammalian CCR4 is encoded by Cnot6 or Cnot6l paralogs. We show that Cnot6l apparently supplies the majority of CCR4 in the maternal CCR4-NOT in mouse, hamster, and bovine oocytes. Deletion of Cnot6l yielded viable mice, but Cnot6l-/- females exhibited ∼40% smaller litter size. The main onset of the phenotype was post-zygotic: fertilized Cnot6l-/- eggs developed slower and arrested more frequently than Cnot6l+/- eggs, suggesting that maternal CNOT6L is necessary for accurate oocyte-to-embryo transition. Transcriptome analysis revealed major transcriptome changes in Cnot6l-/- ovulated eggs and one-cell zygotes. In contrast, minimal transcriptome changes in preovulatory Cnot6l-/- oocytes were consistent with reported Cnot6l mRNA dormancy. A minimal overlap between transcripts sensitive to decapping inhibition and Cnot6l loss suggests that decapping and CNOT6L-mediated deadenylation selectively target distinct subsets of mRNAs during oocyte-to-embryo transition in mouse.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19001529
003      
CZ-PrNML
005      
20190129091104.0
007      
ta
008      
190107s2018 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.26508/lsa.201800084 $2 doi
035    __
$a (PubMed)30456367
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Horvat, Filip $u Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic. Bioinformatics Group, Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
245    10
$a Role of Cnot6l in maternal mRNA turnover / $c F. Horvat, H. Fulka, R. Jankele, R. Malik, M. Jun, K. Solcova, R. Sedlacek, K. Vlahovicek, RM. Schultz, P. Svoboda,
520    9_
$a Removal of poly(A) tail is an important mechanism controlling eukaryotic mRNA turnover. The major eukaryotic deadenylase complex CCR4-NOT contains two deadenylase components, CCR4 and CAF1, for which mammalian CCR4 is encoded by Cnot6 or Cnot6l paralogs. We show that Cnot6l apparently supplies the majority of CCR4 in the maternal CCR4-NOT in mouse, hamster, and bovine oocytes. Deletion of Cnot6l yielded viable mice, but Cnot6l-/- females exhibited ∼40% smaller litter size. The main onset of the phenotype was post-zygotic: fertilized Cnot6l-/- eggs developed slower and arrested more frequently than Cnot6l+/- eggs, suggesting that maternal CNOT6L is necessary for accurate oocyte-to-embryo transition. Transcriptome analysis revealed major transcriptome changes in Cnot6l-/- ovulated eggs and one-cell zygotes. In contrast, minimal transcriptome changes in preovulatory Cnot6l-/- oocytes were consistent with reported Cnot6l mRNA dormancy. A minimal overlap between transcripts sensitive to decapping inhibition and Cnot6l loss suggests that decapping and CNOT6L-mediated deadenylation selectively target distinct subsets of mRNAs during oocyte-to-embryo transition in mouse.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Fulka, Helena $u Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic. Institute of Animal Science, Prague, Czech Republic.
700    1_
$a Jankele, Radek $u Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Malik, Radek $u Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Jun, Ma $u Department of Biology, University of Pennsylvania, Philadelphia, PA, USA. Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA, USA.
700    1_
$a Solcova, Katerina $u Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Sedlacek, Radislav $u Czech Centre for Phenogenomics and Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the Czech Academy of Sciences, v. v. i., Vestec, Czech Republic.
700    1_
$a Vlahovicek, Kristian $u Bioinformatics Group, Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
700    1_
$a Schultz, Richard M $u Department of Biology, University of Pennsylvania, Philadelphia, PA, USA. Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA, USA.
700    1_
$a Svoboda, Petr $u Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
773    0_
$w MED00197314 $t Life science alliance $x 2575-1077 $g Roč. 1, č. 4 (2018), s. e201800084
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30456367 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190129091333 $b ABA008
999    __
$a ind $b bmc $g 1365348 $s 1039652
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 1 $c 4 $d e201800084 $e 20180716 $i 2575-1077 $m Life science alliance $n Life sci. alliance $x MED00197314
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

Možnosti archivace