• Je něco špatně v tomto záznamu ?

DHA upregulates FADS2 expression in primary cortical astrocytes exposed to vitamin A

B. Dziedzic, D. Bewicz-Binkowska, E. Zgorzynska, D. Stulczewski, L. Wieteska, B. Kaza, A. Walczewska

. 2018 ; 67 (4) : 663-668. [pub] 20180510

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19004010

The fads2 gene encoding delta6-desaturase, the rate-limiting enzyme of the LCPUFA biosynthesis is expressed in astrocytes. Dietary fatty acids, which cross the blood-brain barrier, may regulate the transcription of lipogenic enzymes through activation of transcription factors such as peroxisome proliferator-activated receptors (PPARs). The PPARs form the transcription complex with retinoid X receptors (RXRs) that are activated by 9-cis retinoic acid, a metabolite of vitamin A (VA). The study examines whether challenge of astrocytes with VA, prior 24-h treatment with palmitic acid (PA), alpha-linolenic acid (ALA) or docosahexaenoic acid (DHA) has the effect on the FADS2 expression. RT-qPCR showed that in astrocytes not challenged with VA, PA increased fads2 gene expression and DHA decreased it. However, in VA-primed astrocytes, PA doubled the FADS2 mRNA levels, while DHA increased fads2 gene expression, oppositely to non-primed cells. Furthermore, similar changes were seen in VA-primed astrocytes with regard to delta6-desaturase protein levels following PA and DHA treatment. ALA did not have any effect on the FADS2 mRNA and protein levels in either VA-primed or non-primed astrocytes. These findings indicate that in the presence of vitamin A, DHA upregulates fads2 gene expression in astrocytes.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19004010
003      
CZ-PrNML
005      
20190208091450.0
007      
ta
008      
190124s2018 xr d f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.933708 $2 doi
035    __
$a (PubMed)29750879
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Dziedzic, B. $u Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz, Poland
245    10
$a DHA upregulates FADS2 expression in primary cortical astrocytes exposed to vitamin A / $c B. Dziedzic, D. Bewicz-Binkowska, E. Zgorzynska, D. Stulczewski, L. Wieteska, B. Kaza, A. Walczewska
520    9_
$a The fads2 gene encoding delta6-desaturase, the rate-limiting enzyme of the LCPUFA biosynthesis is expressed in astrocytes. Dietary fatty acids, which cross the blood-brain barrier, may regulate the transcription of lipogenic enzymes through activation of transcription factors such as peroxisome proliferator-activated receptors (PPARs). The PPARs form the transcription complex with retinoid X receptors (RXRs) that are activated by 9-cis retinoic acid, a metabolite of vitamin A (VA). The study examines whether challenge of astrocytes with VA, prior 24-h treatment with palmitic acid (PA), alpha-linolenic acid (ALA) or docosahexaenoic acid (DHA) has the effect on the FADS2 expression. RT-qPCR showed that in astrocytes not challenged with VA, PA increased fads2 gene expression and DHA decreased it. However, in VA-primed astrocytes, PA doubled the FADS2 mRNA levels, while DHA increased fads2 gene expression, oppositely to non-primed cells. Furthermore, similar changes were seen in VA-primed astrocytes with regard to delta6-desaturase protein levels following PA and DHA treatment. ALA did not have any effect on the FADS2 mRNA and protein levels in either VA-primed or non-primed astrocytes. These findings indicate that in the presence of vitamin A, DHA upregulates fads2 gene expression in astrocytes.
650    _2
$a zvířata $7 D000818
650    _2
$a novorozená zvířata $7 D000831
650    _2
$a astrocyty $x účinky léků $x metabolismus $7 D001253
650    _2
$a kultivované buňky $7 D002478
650    _2
$a mozková kůra $x účinky léků $x metabolismus $7 D002540
650    _2
$a kyseliny dokosahexaenové $x farmakologie $7 D004281
650    _2
$a exprese genu $7 D015870
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani Wistar $7 D017208
650    _2
$a stearyl-CoA-desaturasa $x biosyntéza $x genetika $7 D013230
650    _2
$a upregulace $x účinky léků $x fyziologie $7 D015854
650    _2
$a vitamin A $x farmakologie $7 D014801
655    _2
$a časopisecké články $7 D016428
700    1_
$a Bewicz-Binkowska, D. $u Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz, Poland
700    1_
$a Zgorzynska, E. $u Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz, Poland
700    1_
$a Stulczewski, D. $u Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz, Poland
700    1_
$a Wieteska, L. $u Department of Medical Biochemistry, Medical University of Lodz, Lodz, Poland
700    1_
$a Kaza, B. $u Laboratory of Molecular Neurobiology, Neurobiology Center, The Nencki Institute of Experimental Biology, Warsaw, Poland
700    1_
$a Walczewska, A. $u Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz, Poland
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 67, č. 4 (2018), s. 663-668
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29750879 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y 4 $z 0
990    __
$a 20190124 $b ABA008
991    __
$a 20190208084644 $b ABA008
999    __
$a ok $b bmc $g 1374799 $s 1042188
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 67 $c 4 $d 663-668 $e 20180510 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK118 $a Pubmed-20190124

Najít záznam

Citační ukazatele

Možnosti archivace