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Workflow for bioprinting of cell-laden bioink
Sebastian Allig, Margot Mayer, Christiane Thielemann
Language English Country Czech Republic
- MeSH
- Biocompatible Materials chemical synthesis isolation & purification therapeutic use MeSH
- Biomedical Technology MeSH
- Bioprinting * methods instrumentation MeSH
- Cell Culture Techniques MeSH
- Microscopy, Fluorescence MeSH
- Humans MeSH
- Organ Culture Techniques MeSH
- In Vitro Techniques MeSH
- Tissue Engineering * methods instrumentation MeSH
- Check Tag
- Humans MeSH
Applying technologies of additive manufacturing to the field of tissue engineering created a pioneering new approach to model complex cell systems artificially. Regarding its huge potential, bioprinting is still in its infancies and many questions are still unanswered. To address this issue, an extrusion-based bioprinting (EBB) process was used to deposit human embryonic kidney (HEK) cells in a defined pattern. It was shown that the bioprinted construct featured a high degree in viability reaching up to 77% 10 days after printing (DAP). This work displays a proof of principle for a controlled cell formation which shall later be applied to in vitro drug screening tests using various types of cells.
Literatura
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