-
Something wrong with this record ?
Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity
E. Nehnevajova, E. Ramireddy, A. Stolz, M. Gerdemann-Knörck, O. Novák, M. Strnad, T. Schmülling,
Language English Country England, Great Britain
Document type Journal Article
NLK
BioMedCentral
from 2001-12-01
BioMedCentral Open Access
from 2001
Directory of Open Access Journals
from 2001
Free Medical Journals
from 2001
PubMed Central
from 2001
Europe PubMed Central
from 2001
ProQuest Central
from 2009-01-01
Open Access Digital Library
from 2001-01-01
Open Access Digital Library
from 2001-09-01
Open Access Digital Library
from 2001-01-01
Medline Complete (EBSCOhost)
from 2001-01-01
Health & Medicine (ProQuest)
from 2009-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2001
Springer Nature OA/Free Journals
from 2001-12-01
- MeSH
- Biodegradation, Environmental * MeSH
- Brassica napus genetics growth & development metabolism MeSH
- Chlorophyll analysis metabolism MeSH
- Cytokinins genetics metabolism MeSH
- Plants, Genetically Modified MeSH
- Cadmium analysis metabolism MeSH
- Plant Roots growth & development metabolism MeSH
- Plant Leaves chemistry metabolism MeSH
- Oxidoreductases genetics metabolism MeSH
- Soil chemistry MeSH
- Zinc analysis metabolism MeSH
- Nutrients analysis metabolism MeSH
- Publication type
- Journal Article MeSH
BACKGROUND: Cytokinin is a negative regulator of root growth, and a reduction of the cytokinin content or signalling causes the formation a larger root system in model plants, improves their growth under drought and nutrient limitation and causes increased accumulation of elements in the shoot. Roots are an important but understudied target of plant breeding. Here we have therefore explored whether root enhancement by lowering the cytokinin content can also be achieved in oilseed rape (Brassica napus L.) plants. RESULTS: Transgenic plants overexpressing the CKX2 gene of Arabidopsis thaliana encoding a cytokinin-degrading cytokinin oxidase/dehydrogenase showed higher CKX activity and a strongly reduced cytokinin content. Cytokinin deficiency led to the formation of a larger root system under different growth conditions, which was mainly due to an increased number of lateral and adventitious roots. In contrast, shoot growth was comparable to wild type, which caused an enhanced root-to-shoot ratio. Transgenic plants accumulated in their leaves higher concentrations of macro- and microelements including P, Ca, Mg, S, Zn, Cu, Mo and Mn. They formed more chlorophyll under Mg- and S-deficiency and accumulated a larger amount of Cd and Zn from contaminated medium and soil. CONCLUSIONS: These findings demonstrate the usefulness of ectopic CKX gene expression to achieve root enhancement in oilseed rape and underpin the functional relevance of a larger root system. Furthermore, the lack of major developmental consequences on shoot growth in cytokinin-deficient oilseed rape indicates species-specific differences of CKX gene and/or cytokinin action.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19011916
- 003
- CZ-PrNML
- 005
- 20190411092901.0
- 007
- ta
- 008
- 190405s2019 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1186/s12870-019-1657-6 $2 doi
- 035 __
- $a (PubMed)30786853
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Nehnevajova, Erika $u Institute of Biology/Applied Genetics, Dahlem Centre of Plant Sciences (DCPS), Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195, Berlin, Germany.
- 245 10
- $a Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity / $c E. Nehnevajova, E. Ramireddy, A. Stolz, M. Gerdemann-Knörck, O. Novák, M. Strnad, T. Schmülling,
- 520 9_
- $a BACKGROUND: Cytokinin is a negative regulator of root growth, and a reduction of the cytokinin content or signalling causes the formation a larger root system in model plants, improves their growth under drought and nutrient limitation and causes increased accumulation of elements in the shoot. Roots are an important but understudied target of plant breeding. Here we have therefore explored whether root enhancement by lowering the cytokinin content can also be achieved in oilseed rape (Brassica napus L.) plants. RESULTS: Transgenic plants overexpressing the CKX2 gene of Arabidopsis thaliana encoding a cytokinin-degrading cytokinin oxidase/dehydrogenase showed higher CKX activity and a strongly reduced cytokinin content. Cytokinin deficiency led to the formation of a larger root system under different growth conditions, which was mainly due to an increased number of lateral and adventitious roots. In contrast, shoot growth was comparable to wild type, which caused an enhanced root-to-shoot ratio. Transgenic plants accumulated in their leaves higher concentrations of macro- and microelements including P, Ca, Mg, S, Zn, Cu, Mo and Mn. They formed more chlorophyll under Mg- and S-deficiency and accumulated a larger amount of Cd and Zn from contaminated medium and soil. CONCLUSIONS: These findings demonstrate the usefulness of ectopic CKX gene expression to achieve root enhancement in oilseed rape and underpin the functional relevance of a larger root system. Furthermore, the lack of major developmental consequences on shoot growth in cytokinin-deficient oilseed rape indicates species-specific differences of CKX gene and/or cytokinin action.
- 650 12
- $a biodegradace $7 D001673
- 650 _2
- $a Brassica napus $x genetika $x růst a vývoj $x metabolismus $7 D029688
- 650 _2
- $a kadmium $x analýza $x metabolismus $7 D002104
- 650 _2
- $a chlorofyl $x analýza $x metabolismus $7 D002734
- 650 _2
- $a cytokininy $x genetika $x metabolismus $7 D003583
- 650 _2
- $a živiny $x analýza $x metabolismus $7 D000078622
- 650 _2
- $a oxidoreduktasy $x genetika $x metabolismus $7 D010088
- 650 _2
- $a listy rostlin $x chemie $x metabolismus $7 D018515
- 650 _2
- $a kořeny rostlin $x růst a vývoj $x metabolismus $7 D018517
- 650 _2
- $a geneticky modifikované rostliny $7 D030821
- 650 _2
- $a půda $x chemie $7 D012987
- 650 _2
- $a zinek $x analýza $x metabolismus $7 D015032
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Ramireddy, Eswarayya $u Institute of Biology/Applied Genetics, Dahlem Centre of Plant Sciences (DCPS), Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195, Berlin, Germany. Present address: Indian Institute of Science Education and Research (IISER) Tirupati, Biology Division, 517507, Tirupati, Andhra Pradesh, India.
- 700 1_
- $a Stolz, Andrea $u Institute of Biology/Applied Genetics, Dahlem Centre of Plant Sciences (DCPS), Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195, Berlin, Germany.
- 700 1_
- $a Gerdemann-Knörck, Maria $u Institute of Biology/Applied Genetics, Dahlem Centre of Plant Sciences (DCPS), Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195, Berlin, Germany.
- 700 1_
- $a Novák, Ondřej $u Czech Academy of Sciences, Institute of Experimental Botany & Palacký University, Olomouc, Czech Republic.
- 700 1_
- $a Strnad, Miroslav $u Czech Academy of Sciences, Institute of Experimental Botany & Palacký University, Olomouc, Czech Republic.
- 700 1_
- $a Schmülling, Thomas $u Institute of Biology/Applied Genetics, Dahlem Centre of Plant Sciences (DCPS), Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195, Berlin, Germany. tschmue@zedat.fu-berlin.de.
- 773 0_
- $w MED00006798 $t BMC plant biology $x 1471-2229 $g Roč. 19, č. 1 (2019), s. 83
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30786853 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190405 $b ABA008
- 991 __
- $a 20190411092918 $b ABA008
- 999 __
- $a ok $b bmc $g 1391226 $s 1050221
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 19 $c 1 $d 83 $e 20190220 $i 1471-2229 $m Bmc plant biology $n BMC Plant Biol $x MED00006798
- LZP __
- $a Pubmed-20190405
