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Application of HPCCC Combined with Polymeric Resins and HPLC for the Separation of Cyclic Lipopeptides Muscotoxins A⁻C and Their Antimicrobial Activity
J. Cheel, J. Hájek, M. Kuzma, K. Saurav, I. Smýkalová, E. Ondráčková, P. Urajová, DL. Vu, K. Faure, J. Kopecký, P. Hrouzek,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Free Medical Journals od 1997
PubMed Central od 2001
Europe PubMed Central od 2001
ProQuest Central od 1997-01-01
Open Access Digital Library od 1997-01-01
Medline Complete (EBSCOhost) od 2009-03-01
Health & Medicine (ProQuest) od 1997-01-01
Odkazy
PubMed
30332796
DOI
10.3390/molecules23102653
Knihovny.cz E-zdroje
- MeSH
- antiinfekční látky chemie izolace a purifikace farmakologie MeSH
- Ascomycota účinky léků MeSH
- Bacillus subtilis účinky léků MeSH
- bakteriální toxiny chemie izolace a purifikace farmakologie MeSH
- cyklické peptidy chemie farmakologie MeSH
- lipopeptidy chemie farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- molekulární struktura MeSH
- sinice metabolismus MeSH
- syntetické pryskyřice chemie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
Muscotoxins are cyanobacterial cyclic lipopeptides with potential applications in biomedicine and biotechnology. In this study, Desmonostoc muscorum CCALA125 strain extracts were enriched by polymeric resin treatment, and subjected to HPCCC affording three cyclic lipopeptides (1⁻3), which were further repurified by semi-preparative HPLC, affording 1, 2, and 3, with a purity of 86%, 92%, and 90%, respectively. The chemical identities of 2⁻3 were determined as muscotoxins A and B, respectively, by comparison with previously reported ESI-HRMS/MS data, whereas 1 was determined as a novel muscotoxin variant (muscotoxin C) using NMR and ESI-HRMS/MS data. Owing to the high yield (50 mg), compound 2 was broadly screened for its antimicrobial potential exhibiting a strong antifungal activity against Alternaria alternata, Monographella cucumerina, and Aspergillus fumigatus, with minimum inhibitory concentration (MIC) values of 0.58, 2.34, and 2.34 µg/mL; respectively, and weak antibacterial activity against Bacillus subtilis with a MIC value of 37.5 µg/mL. Compounds 1 and 3 were tested only against the plant pathogenic fungus Sclerotinia sclerotiorum due to their low yield, displaying a moderate antifungal activity. The developed chromatographic method proved to be an efficient tool for obtaining muscotoxins with potent antifungal properties.
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- $a Cheel, José $u Laboratory of Algal Biotechnology-Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický mlýn, Novohradská 237, 379 81 Třeboň, Czech Republic. jcheel@alga.cz.
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- $a Application of HPCCC Combined with Polymeric Resins and HPLC for the Separation of Cyclic Lipopeptides Muscotoxins A⁻C and Their Antimicrobial Activity / $c J. Cheel, J. Hájek, M. Kuzma, K. Saurav, I. Smýkalová, E. Ondráčková, P. Urajová, DL. Vu, K. Faure, J. Kopecký, P. Hrouzek,
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- $a Muscotoxins are cyanobacterial cyclic lipopeptides with potential applications in biomedicine and biotechnology. In this study, Desmonostoc muscorum CCALA125 strain extracts were enriched by polymeric resin treatment, and subjected to HPCCC affording three cyclic lipopeptides (1⁻3), which were further repurified by semi-preparative HPLC, affording 1, 2, and 3, with a purity of 86%, 92%, and 90%, respectively. The chemical identities of 2⁻3 were determined as muscotoxins A and B, respectively, by comparison with previously reported ESI-HRMS/MS data, whereas 1 was determined as a novel muscotoxin variant (muscotoxin C) using NMR and ESI-HRMS/MS data. Owing to the high yield (50 mg), compound 2 was broadly screened for its antimicrobial potential exhibiting a strong antifungal activity against Alternaria alternata, Monographella cucumerina, and Aspergillus fumigatus, with minimum inhibitory concentration (MIC) values of 0.58, 2.34, and 2.34 µg/mL; respectively, and weak antibacterial activity against Bacillus subtilis with a MIC value of 37.5 µg/mL. Compounds 1 and 3 were tested only against the plant pathogenic fungus Sclerotinia sclerotiorum due to their low yield, displaying a moderate antifungal activity. The developed chromatographic method proved to be an efficient tool for obtaining muscotoxins with potent antifungal properties.
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- $a Kuzma, Marek $u Laboratory of Molecular Structure Characterization, Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic. kuzma@biomed.cas.cz.
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- $a Saurav, Kumar $u Laboratory of Algal Biotechnology-Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický mlýn, Novohradská 237, 379 81 Třeboň, Czech Republic. sauravverma17@gmail.com.
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- $a Smýkalová, Iva $u Plant Biotechnology Department, AGRITEC Plant Research Ltd., Zemědělská 2520/16, 787 01 Šumperk, Czech Republic. smykalova@agritec.cz.
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- $a Faure, Karine $u Institut des Sciences Analytiques, University of Lyon, CNRS, Université Claude Bernard Lyon 1, Ens de Lyon, UMR 5280, 5 rue de la Doua, 69100 Villeurbanne, France. karine.faure@isa-lyon.fr.
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- $a Kopecký, Jiří $u Laboratory of Algal Biotechnology-Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický mlýn, Novohradská 237, 379 81 Třeboň, Czech Republic. kopecky@alga.cz.
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- $a Hrouzek, Pavel $u Laboratory of Algal Biotechnology-Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický mlýn, Novohradská 237, 379 81 Třeboň, Czech Republic. hrouzek@alga.cz.
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