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Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes
S. Dixit, J. Lukeš,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1995 to 6 months ago
PubMed Central
from 1995 to 1 year ago
Europe PubMed Central
from 1995 to 1 year ago
Open Access Digital Library
from 1995-03-01
- MeSH
- RNA Editing MeSH
- RNA, Messenger genetics metabolism MeSH
- Mitochondria genetics metabolism MeSH
- RNA-Binding Proteins metabolism MeSH
- Protozoan Proteins genetics metabolism MeSH
- RNA, Mitochondrial genetics metabolism MeSH
- Trypanosoma genetics metabolism MeSH
- Protein Binding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNAs, while TbRGG2 is required for its efficient progression on pan-edited mRNAs. Both MRP1/2 and TbRGG2 are capable of modulating RNA-RNA interactions in vitro. As determined by using iCLIP and RIP-qPCR, RNAs bound to MRP1/2 are characterized and compared with those associated with MRB8170 and TbRGG2. We provide evidence that MRP1 and MRB8170 have correlated binding and similar RNA crosslinking peak profiles over minimally and never-edited mRNAs. Our results suggest that MRP1 assists MRB8170 in RNA editing on minimally edited mRNAs.
References provided by Crossref.org
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