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Exocyst Subunit EXO70H4 Has a Specific Role in Callose Synthase Secretion and Silica Accumulation
I. Kulich, Z. Vojtíková, P. Sabol, J. Ortmannová, V. Neděla, E. Tihlaříková, V. Žárský,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
E-resources NLK Online Full text
Free Medical Journals from 1926 to 1 year agoOpen Access Digital Library from 1926-01-01
Links
PubMed
29301954
DOI
10.1104/pp.17.01693
Knihovny.cz E-resources
- MeSH
- Arabidopsis drug effects genetics metabolism MeSH
- Cell Membrane drug effects metabolism MeSH
- Cell Wall drug effects metabolism MeSH
- Plant Epidermis cytology drug effects metabolism MeSH
- Phenotype MeSH
- Flagellin pharmacology MeSH
- Glucans MeSH
- Glucosyltransferases metabolism MeSH
- Mutation genetics MeSH
- Silicon Dioxide metabolism MeSH
- Protein Subunits chemistry metabolism MeSH
- Protein Domains MeSH
- Arabidopsis Proteins chemistry metabolism MeSH
- Gene Expression Regulation, Plant drug effects MeSH
- Trichomes metabolism MeSH
- Up-Regulation drug effects MeSH
- Vesicular Transport Proteins chemistry metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Biogenesis of the plant secondary cell wall involves many important aspects, such as phenolic compound deposition and often silica encrustation. Previously, we demonstrated the importance of the exocyst subunit EXO70H4 for biogenesis of the trichome secondary cell wall, namely for deposition of the autofluorescent and callose-rich cell wall layer. Here, we reveal that EXO70H4-driven cell wall biogenesis is constitutively active in the mature trichome, but also can be activated elsewhere upon pathogen attack, giving this study a broader significance with an overlap into phytopathology. To address the specificity of EXO70H4 among the EXO70 family, we complemented the exo70H4-1 mutant by 18 different Arabidopsis (Arabidopsis thaliana) EXO70 paralogs subcloned under the EXO70H4 promoter. Only EXO70H4 had the capacity to rescue the exo70H4-1 trichome phenotype. Callose deposition phenotype of exo70H4-1 mutant is caused by impaired secretion of PMR4, a callose synthase responsible for the synthesis of callose in the trichome. PMR4 colocalizes with EXO70H4 on plasma membrane microdomains that do not develop in the exo70H4-1 mutant. Using energy-dispersive x-ray microanalysis, we show that both EXO70H4- and PMR4-dependent callose deposition in the trichome are essential for cell wall silicification.
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- $a Kulich, Ivan $u Department of Experimental Plant Biology, Faculty of Sciences, Charles University, Prague, Czech Republic kulich@natur.cuni.cz.
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- $a Biogenesis of the plant secondary cell wall involves many important aspects, such as phenolic compound deposition and often silica encrustation. Previously, we demonstrated the importance of the exocyst subunit EXO70H4 for biogenesis of the trichome secondary cell wall, namely for deposition of the autofluorescent and callose-rich cell wall layer. Here, we reveal that EXO70H4-driven cell wall biogenesis is constitutively active in the mature trichome, but also can be activated elsewhere upon pathogen attack, giving this study a broader significance with an overlap into phytopathology. To address the specificity of EXO70H4 among the EXO70 family, we complemented the exo70H4-1 mutant by 18 different Arabidopsis (Arabidopsis thaliana) EXO70 paralogs subcloned under the EXO70H4 promoter. Only EXO70H4 had the capacity to rescue the exo70H4-1 trichome phenotype. Callose deposition phenotype of exo70H4-1 mutant is caused by impaired secretion of PMR4, a callose synthase responsible for the synthesis of callose in the trichome. PMR4 colocalizes with EXO70H4 on plasma membrane microdomains that do not develop in the exo70H4-1 mutant. Using energy-dispersive x-ray microanalysis, we show that both EXO70H4- and PMR4-dependent callose deposition in the trichome are essential for cell wall silicification.
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- $a Vojtíková, Zdeňka $u Department of Experimental Plant Biology, Faculty of Sciences, Charles University, Prague, Czech Republic.
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- $a Sabol, Peter $u Department of Experimental Plant Biology, Faculty of Sciences, Charles University, Prague, Czech Republic.
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- $a Žárský, Viktor $u Department of Experimental Plant Biology, Faculty of Sciences, Charles University, Prague, Czech Republic. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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