-
Je něco špatně v tomto záznamu ?
The polymorphic insertion of the luteinizing hormone receptor "insLQ" show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles
T. Borgbo, J. Chrudimska, M. Macek, JV. Jeppesen, JA. Bøtkjær, SG. Kristensen, KT. Macklon, E. Ernst, LL. Hansen, C. Yding Andersen,
Jazyk angličtina Země Irsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- dospělí MeSH
- folikulární buňky metabolismus MeSH
- folikulární tekutina metabolismus MeSH
- genotyp MeSH
- hormony metabolismus MeSH
- inzerční mutageneze * MeSH
- lidé MeSH
- messenger RNA genetika metabolismus MeSH
- mladiství MeSH
- mladý dospělý MeSH
- ovariální folikul metabolismus MeSH
- polymorfismus genetický * MeSH
- receptory LH genetika MeSH
- regulace genové exprese * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to -/insLQ and the -/- genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in -/insLQ compared to the -/- genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.
Department of Biomedicine University of Aarhus Aarhus Denmark
Laboratory of Reproductive Biology Copenhagen University Hospital Rigshospitalet Copenhagen Denmark
The Fertility Clinic Aarhus University Hospital Aarhus Denmark
The Fertility Clinic Copenhagen University Hospital Rigshospitalet Copenhagen Denmark
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19013135
- 003
- CZ-PrNML
- 005
- 20190416120722.0
- 007
- ta
- 008
- 190405s2018 ie f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.mce.2017.07.002 $2 doi
- 035 __
- $a (PubMed)28684292
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ie
- 100 1_
- $a Borgbo, T $u Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark; Department of Biomedicine, University of Aarhus, Aarhus, Denmark.
- 245 14
- $a The polymorphic insertion of the luteinizing hormone receptor "insLQ" show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles / $c T. Borgbo, J. Chrudimska, M. Macek, JV. Jeppesen, JA. Bøtkjær, SG. Kristensen, KT. Macklon, E. Ernst, LL. Hansen, C. Yding Andersen,
- 520 9_
- $a The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to -/insLQ and the -/- genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in -/insLQ compared to the -/- genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.
- 650 _2
- $a mladiství $7 D000293
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a folikulární tekutina $x metabolismus $7 D015571
- 650 12
- $a regulace genové exprese $7 D005786
- 650 _2
- $a genotyp $7 D005838
- 650 _2
- $a folikulární buňky $x metabolismus $7 D006107
- 650 _2
- $a hormony $x metabolismus $7 D006728
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a inzerční mutageneze $7 D016254
- 650 _2
- $a ovariální folikul $x metabolismus $7 D006080
- 650 12
- $a polymorfismus genetický $7 D011110
- 650 _2
- $a messenger RNA $x genetika $x metabolismus $7 D012333
- 650 _2
- $a receptory LH $x genetika $7 D011974
- 650 _2
- $a mladý dospělý $7 D055815
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Chrudimska, J $u Department of Biology and Medical Genetics, 2nd Faculty of Medicine Charles University, University Hospital Motol, Prague, Czech Republic.
- 700 1_
- $a Macek, M $u Department of Biology and Medical Genetics, 2nd Faculty of Medicine Charles University, University Hospital Motol, Prague, Czech Republic.
- 700 1_
- $a Jeppesen, J V $u Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
- 700 1_
- $a Bøtkjær, J A $u Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
- 700 1_
- $a Kristensen, S G $u Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
- 700 1_
- $a Macklon, K T $u The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
- 700 1_
- $a Ernst, E $u The Fertility Clinic, Aarhus University Hospital, Aarhus, Denmark.
- 700 1_
- $a Hansen, L L $u Department of Biomedicine, University of Aarhus, Aarhus, Denmark.
- 700 1_
- $a Yding Andersen, C $u Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark. Electronic address: claus.yding.andersen@regionh.dk.
- 773 0_
- $w MED00003387 $t Molecular and cellular endocrinology $x 1872-8057 $g Roč. 460, č. - (2018), s. 57-62
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28684292 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190405 $b ABA008
- 991 __
- $a 20190416120747 $b ABA008
- 999 __
- $a ok $b bmc $g 1392445 $s 1051440
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 460 $c - $d 57-62 $e 20170703 $i 1872-8057 $m Molecular and cellular endocrinology $n Mol Cell Endocrinol $x MED00003387
- LZP __
- $a Pubmed-20190405
