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Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry
E. Gerdtsson, M. Pore, JA. Thiele, AS. Gerdtsson, PD. Malihi, R. Nevarez, A. Kolatkar, CR. Velasco, S. Wix, M. Singh, A. Carlsson, AJ. Zurita, C. Logothetis, AA. Merchant, J. Hicks, P. Kuhn,
Convergent science physical oncology.
2018 ;
4 (1) :
.
[pub] 20180116
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
Perzistentní odkaz
https://www.medvik.cz/link/bmc19013220
PubMed
30906572
DOI
10.1088/2057-1739/aaa013
Knihovny.cz E-zdroje
- Publikační typ
- časopisecké články MeSH
Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.
Citace poskytuje Crossref.org
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- $a Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.
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- $a Pore, Milind $u Bridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
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- $a Thiele, Jana-Aletta $u Biomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, 323 00 Pilsen, Czech Republic; Jana.A.Thiele@lfp.cuni.cz.
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- $a Gerdtsson, Anna Sandström $u Bridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
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- $a Malihi, Paymaneh D $u Bridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
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- $a Nevarez, Rafael $u Bridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
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- $a Kolatkar, Anand $u Bridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
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- $a Wix, Sophia $u Bridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
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- $a Singh, Mohan $u Division of Hematology, Department of Medicine, USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, 1450 Biggy Street, Los Angeles, CA; mohansin@usc.edu; akilmerc@usc.edu.
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- $a Carlsson, Anders $u Bridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
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- $a Zurita, Amado J $u Department of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Unit 207, Houston, Texas; azurita@mdanderson.org; clogothe@mdanderson.org.
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