• Je něco špatně v tomto záznamu ?

Tyrosine kinase Pyk2 is involved in colonic smooth muscle contraction via the RhoA/ROCK pathway

Ling Tong, Juan-Ping Ao, Hong-Li Lu, Xu Huang, Jing-Yu Zang, Shao-Hua Liu, Ni-Na Song, Shi-Qi Huang, Chen Lu, Jie Chen, Wen-Xie Xu

. 2019 ; 68 (1) : 89-98. [pub] 20181023

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19024859

The contraction of gastrointestinal (GI) smooth muscles is regulated by both Ca(2+)-dependent and Ca(2+) sensitization mechanisms. Proline-rich tyrosine kinase 2 (Pyk2) is involved in the depolarization-induced contraction of vascular smooth muscle via a Ca(2+) sensitization pathway. However, the role of Pyk2 in GI smooth muscle contraction is unclear. The spontaneous contraction of colonic smooth muscle was measured by using isometric force transducers. Protein and phosphorylation levels were determined by using western blotting. Pyk2 protein was expressed in colonic tissue, and spontaneous colonic contractions were inhibited by PF-431396, a Pyk2 inhibitor, in the presence of tetrodotoxin (TTX). In cultured colonic smooth muscle cells (CSMCs), PF-431396 decreased the levels of myosin light chain (MLC20) phosphorylated at Ser19 and ROCK2 protein expression, but myosin light chain kinase (MLCK) expression was not altered. However, Y-27632, a Rho kinase inhibitor, increased phosphorylation of Pyk2 at Tyr402 and concomitantly decreased ROCK2 levels; the expression of MLCK in CSMCs did not change. The expression of P(Tyr402)-Pyk2 and ROCK2 was increased when CSMCs were treated with Ach. Pyk2 is involved in the process of colonic smooth muscle contraction through the RhoA/ROCK pathway. These pathways may provide very important targets for investigating GI motility disorders.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19024859
003      
CZ-PrNML
005      
20190805125504.0
007      
ta
008      
190704s2019 xr d f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.933857 $2 doi
035    __
$a (PubMed)30433799
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Tong, Ling $u Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Pediatric Surgery, Xin Hua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, China
245    10
$a Tyrosine kinase Pyk2 is involved in colonic smooth muscle contraction via the RhoA/ROCK pathway / $c Ling Tong, Juan-Ping Ao, Hong-Li Lu, Xu Huang, Jing-Yu Zang, Shao-Hua Liu, Ni-Na Song, Shi-Qi Huang, Chen Lu, Jie Chen, Wen-Xie Xu
520    9_
$a The contraction of gastrointestinal (GI) smooth muscles is regulated by both Ca(2+)-dependent and Ca(2+) sensitization mechanisms. Proline-rich tyrosine kinase 2 (Pyk2) is involved in the depolarization-induced contraction of vascular smooth muscle via a Ca(2+) sensitization pathway. However, the role of Pyk2 in GI smooth muscle contraction is unclear. The spontaneous contraction of colonic smooth muscle was measured by using isometric force transducers. Protein and phosphorylation levels were determined by using western blotting. Pyk2 protein was expressed in colonic tissue, and spontaneous colonic contractions were inhibited by PF-431396, a Pyk2 inhibitor, in the presence of tetrodotoxin (TTX). In cultured colonic smooth muscle cells (CSMCs), PF-431396 decreased the levels of myosin light chain (MLC20) phosphorylated at Ser19 and ROCK2 protein expression, but myosin light chain kinase (MLCK) expression was not altered. However, Y-27632, a Rho kinase inhibitor, increased phosphorylation of Pyk2 at Tyr402 and concomitantly decreased ROCK2 levels; the expression of MLCK in CSMCs did not change. The expression of P(Tyr402)-Pyk2 and ROCK2 was increased when CSMCs were treated with Ach. Pyk2 is involved in the process of colonic smooth muscle contraction through the RhoA/ROCK pathway. These pathways may provide very important targets for investigating GI motility disorders.
650    _2
$a zvířata $7 D000818
650    _2
$a buněčné kultury $7 D018929
650    _2
$a kolon $x účinky léků $x metabolismus $7 D003106
650    _2
$a inhibitory enzymů $x farmakologie $7 D004791
650    _2
$a fokální adhezní kinasa 2 $x antagonisté a inhibitory $x biosyntéza $7 D051418
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední ICR $7 D008813
650    _2
$a svalová kontrakce $x účinky léků $x fyziologie $7 D009119
650    _2
$a hladké svalstvo $x účinky léků $x metabolismus $7 D009130
650    _2
$a orgánové kultury - kultivační techniky $7 D009924
650    _2
$a signální transdukce $x účinky léků $x fyziologie $7 D015398
650    _2
$a rho proteiny vázající GTP $x biosyntéza $7 D020741
650    _2
$a kinázy asociované s rho $x biosyntéza $7 D054460
655    _2
$a časopisecké články $7 D016428
700    1_
$a Ao, Jun-Ping $u State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
700    1_
$a Lu, Hong-Li $u Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
700    1_
$a Huang, Xu $u Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
700    1_
$a Zang, Jing-Yu $u Department of Pediatric Surgery, Xin Hua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, China
700    1_
$a Liu, Shao-Hua $u Department of Anesthesiology, Soth Renji Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, China
700    1_
$a Song, Ni-Nn $u Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
700    1_
$a Huang, Shi-Qi $u Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
700    1_
$a Lu, Chen $u Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
700    1_
$a Chen, Jie $u State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
700    1_
$a Xu, Wen-Xie $u Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Pediatric Surgery, Xin Hua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, China
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 68, č. 1 (2019), s. 89-98
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30433799 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y 4 $z 0
990    __
$a 20190704 $b ABA008
991    __
$a 20190730135729 $b ABA008
999    __
$a ok $b bmc $g 1429482 $s 1063277
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 68 $c 1 $d 89-98 $e 20181023 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK111 $a Pubmed-20190704

Najít záznam

Citační ukazatele

Možnosti archivace