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Isolation and characterization of CAC antenna proteins and photosystem I supercomplex from the cryptophytic alga Rhodomonas salina
E. Kuthanová Trsková, D. Bína, S. Santabarbara, R. Sobotka, R. Kaňa, E. Belgio,
Jazyk angličtina Země Dánsko
Typ dokumentu časopisecké články
Grantová podpora
MSMT LO1416
Czech Ministry of Education, Youth and Sport
17-02363Y
Czech Science Foundation
PubMed
30677144
DOI
10.1111/ppl.12928
Knihovny.cz E-zdroje
- MeSH
- chlorofyl metabolismus MeSH
- fluorescenční spektrometrie MeSH
- fotosystém I (proteinový komplex) metabolismus MeSH
- Rhodophyta metabolismus MeSH
- xanthofyly metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
In the present paper, we report an improved method combining sucrose density gradient with ion-exchange chromatography for the isolation of pure chlorophyll a/c antenna proteins from the model cryptophytic alga Rhodomonas salina. Antennas were used for in vitro quenching experiments in the absence of xanthophylls, showing that protein aggregation is a plausible mechanism behind non-photochemical quenching in R. salina. From sucrose gradient, it was also possible to purify a functional photosystem I supercomplex, which was in turn characterized by steady-state and time-resolved fluorescence spectroscopy. R. salina photosystem I showed a remarkably fast photochemical trapping rate, similar to what recently reported for other red clade algae such as Chromera velia and Phaeodactylum tricornutum. The method reported therefore may also be suitable for other still partially unexplored algae, such as cryptophytes.
Citace poskytuje Crossref.org
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