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Fluorescence assay to predict activity of the glycopeptide antibiotics

V. Vimberg, R. Gazak, Z. Szűcs, A. Borbás, P. Herczegh, JP. Cavanagh, L. Zieglerova, J. Závora, V. Adámková, G. Balikova Novotna,

. 2019 ; 72 (2) : 114-117. [pub] 20181130

Language English Country Japan

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc19028012

Grant support
NV15-28807A MZ0 CEP Register

Here, we describe a fluorescent assay developed to study competitive binding of the glycopeptide antibiotics to live bacteria cells. This assay demonstrated that the mechanism of action of the lipoglycopeptide antibiotics strongly depends on the hydrophobicity of the substitutes, with the best antibacterial activity of the glycopeptide antibiotics equally sharing properties of binding to D-Ala-D-Ala residues of the nascent peptidoglycan and to the membrane.

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$a Vimberg, Vladimir $u Institute of Microbiology v. v. i., Czech Academy of Sciences, Vestec, Czech Republic. vladimir.vimberg@biomed.cas.cz.
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$a Gazak, Radek $u Institute of Microbiology v. v. i., Czech Academy of Sciences, Vestec, Czech Republic.
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$a Szűcs, Zsolt $u Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen, Hungary.
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$a Borbás, Aniko $u Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen, Hungary.
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$a Herczegh, Pal $u Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen, Hungary.
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$a Cavanagh, Jorunn Pauline $u Department of Pediatrics, University Hospital of North Norway, Tromsø, Norway. Department of Clinical Medicine, UiT The Arctic University of Norway, Tromsø, Norway.
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$a Zieglerova, Leona $u Institute of Microbiology v. v. i., Czech Academy of Sciences, Vestec, Czech Republic.
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$a Závora, Jan $u Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, Prague, Czech Republic.
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$a Adámková, Václava $u Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, Prague, Czech Republic.
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