-
Je něco špatně v tomto záznamu ?
Butyrate interacts with benzo[a]pyrene to alter expression and activities of xenobiotic metabolizing enzymes involved in metabolism of carcinogens within colon epithelial cell models
O. Zapletal, J. Procházková, V. Dubec, J. Hofmanová, A. Kozubík, J. Vondráček,
Jazyk angličtina Země Irsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- benzopyren toxicita MeSH
- buněčné linie MeSH
- butyráty farmakologie MeSH
- epitelové buňky účinky léků metabolismus MeSH
- karcinogeny toxicita MeSH
- kolon cytologie MeSH
- lidé MeSH
- oxidoreduktasy genetika metabolismus MeSH
- transferasy genetika metabolismus MeSH
- xenobiotika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Butyrate helps to maintain colon homeostasis and exhibits chemopreventive effects in colon epithelium. We examined the interactive effects of butyrate and benzo[a]pyrene (BaP), dietary carcinogen, in regulation of expression of a panel of phase I and II xenobiotic metabolizing enzymes (XMEs) in human colon cells. In human colon carcinoma HCT-116 and HT-29 cell lines, butyrate alone increased mRNA levels of some enzymes, such as N-acetyltransferases (in particular NAT2). In combination with BaP, butyrate potentiated induction of cytochrome P450 family 1 enzymes (CYP1A1), aldo-keto reductases (AKR1C1) or UDP-glucuronosyltransferases (UGT1A1). There were some notable differences between cell lines, as butyrate potentiated induction of NAD(P)H:quinone oxidoreductase 1 (NQO1) and UGT1A4 only in HCT-116 cells, and it even repressed AKR1C3 induction in HT-29 cells. Butyrate also promoted induction of CYP1, NQO1, NAT2, UGT1A1 or UGT1A4 in human colon Caco-2 cells, in a differentiation-dependent manner. Differentiated Caco-2 cells exhibited a higher inducibility of selected XME genes than undifferentiated cells. Butyrate increased induction of enzymatic activities of NATs, NQO1 and UGTs by BaP in HCT-116 and HT29 cells, whereas in differentiated Caco-2 cells it helped to increase only enzymatic activity of NQO1 and UGTs. Together, the present data suggest that butyrate may modulate expression/activities of several enzymes involved in metabolism of carcinogens in colon. In some cases (NAT2, UGT1 A1), this was linked to inhibition of histone deacetylases (HDAC), as confirmed by using HDAC inhibitor trichostatin A. These results may have implications for our understanding of the role of butyrate in regulation of XMEs and carcinogen metabolism in colon.
Department of Chemistry and Toxicology Veterinary Research Institute 62100 Brno Czech Republic
Department of Experimental Biology Faculty of Science Masaryk University 61137 Brno Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19028078
- 003
- CZ-PrNML
- 005
- 20190816124446.0
- 007
- ta
- 008
- 190813s2019 ie f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.tox.2018.11.001 $2 doi
- 035 __
- $a (PubMed)30439556
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ie
- 100 1_
- $a Zapletal, Ondřej $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 61265 Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, 61137 Brno, Czech Republic.
- 245 10
- $a Butyrate interacts with benzo[a]pyrene to alter expression and activities of xenobiotic metabolizing enzymes involved in metabolism of carcinogens within colon epithelial cell models / $c O. Zapletal, J. Procházková, V. Dubec, J. Hofmanová, A. Kozubík, J. Vondráček,
- 520 9_
- $a Butyrate helps to maintain colon homeostasis and exhibits chemopreventive effects in colon epithelium. We examined the interactive effects of butyrate and benzo[a]pyrene (BaP), dietary carcinogen, in regulation of expression of a panel of phase I and II xenobiotic metabolizing enzymes (XMEs) in human colon cells. In human colon carcinoma HCT-116 and HT-29 cell lines, butyrate alone increased mRNA levels of some enzymes, such as N-acetyltransferases (in particular NAT2). In combination with BaP, butyrate potentiated induction of cytochrome P450 family 1 enzymes (CYP1A1), aldo-keto reductases (AKR1C1) or UDP-glucuronosyltransferases (UGT1A1). There were some notable differences between cell lines, as butyrate potentiated induction of NAD(P)H:quinone oxidoreductase 1 (NQO1) and UGT1A4 only in HCT-116 cells, and it even repressed AKR1C3 induction in HT-29 cells. Butyrate also promoted induction of CYP1, NQO1, NAT2, UGT1A1 or UGT1A4 in human colon Caco-2 cells, in a differentiation-dependent manner. Differentiated Caco-2 cells exhibited a higher inducibility of selected XME genes than undifferentiated cells. Butyrate increased induction of enzymatic activities of NATs, NQO1 and UGTs by BaP in HCT-116 and HT29 cells, whereas in differentiated Caco-2 cells it helped to increase only enzymatic activity of NQO1 and UGTs. Together, the present data suggest that butyrate may modulate expression/activities of several enzymes involved in metabolism of carcinogens in colon. In some cases (NAT2, UGT1 A1), this was linked to inhibition of histone deacetylases (HDAC), as confirmed by using HDAC inhibitor trichostatin A. These results may have implications for our understanding of the role of butyrate in regulation of XMEs and carcinogen metabolism in colon.
- 650 _2
- $a benzopyren $x toxicita $7 D001564
- 650 _2
- $a butyráty $x farmakologie $7 D002087
- 650 _2
- $a karcinogeny $x toxicita $7 D002273
- 650 _2
- $a buněčné linie $7 D002460
- 650 _2
- $a kolon $x cytologie $7 D003106
- 650 _2
- $a epitelové buňky $x účinky léků $x metabolismus $7 D004847
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a oxidoreduktasy $x genetika $x metabolismus $7 D010088
- 650 _2
- $a transferasy $x genetika $x metabolismus $7 D014166
- 650 _2
- $a xenobiotika $x metabolismus $7 D015262
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Procházková, Jiřina $u Department of Chemistry and Toxicology, Veterinary Research Institute, 62100 Brno, Czech Republic.
- 700 1_
- $a Dubec, Vít $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 61265 Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, 61137 Brno, Czech Republic.
- 700 1_
- $a Hofmanová, Jiřina $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 61265 Brno, Czech Republic.
- 700 1_
- $a Kozubík, Alois $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 61265 Brno, Czech Republic.
- 700 1_
- $a Vondráček, Jan $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 61265 Brno, Czech Republic. Electronic address: vondracek@ibp.cz.
- 773 0_
- $w MED00004533 $t Toxicology $x 1879-3185 $g Roč. 412, č. - (2019), s. 1-11
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30439556 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190813 $b ABA008
- 991 __
- $a 20190816124716 $b ABA008
- 999 __
- $a ok $b bmc $g 1433227 $s 1066538
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 412 $c - $d 1-11 $e 20181113 $i 1879-3185 $m Toxicology $n Toxicology $x MED00004533
- LZP __
- $a Pubmed-20190813
