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Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase
MS. Proctor, JW. Chidgey, MK. Shukla, PJ. Jackson, R. Sobotka, CN. Hunter, A. Hitchcock,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
BB/M000265/1
Biotechnology and Biological Sciences Research Council - United Kingdom
NLK
Medline Complete (EBSCOhost)
from 2016-05-01 to 1 year ago
Wiley Free Content
from 1997 to 1 year ago
- MeSH
- Bacterial Proteins genetics metabolism MeSH
- Photosynthesis radiation effects MeSH
- Photosystem II Protein Complex genetics metabolism MeSH
- Phylogeny MeSH
- Carbon-Oxygen Ligases classification genetics metabolism MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Light MeSH
- Synechocystis genetics metabolism MeSH
- Thylakoids metabolism radiation effects MeSH
- Protein Binding radiation effects MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
In the model cyanobacterium Synechocystis sp. PCC 6803, the terminal enzyme of chlorophyll biosynthesis, chlorophyll synthase (ChlG), forms a complex with high light-inducible proteins, the photosystem II assembly factor Ycf39 and the YidC/Alb3/OxaI membrane insertase, co-ordinating chlorophyll delivery with cotranslational insertion of nascent photosystem polypeptides into the membrane. To gain insight into the ubiquity of this assembly complex in higher photosynthetic organisms, we produced functional foreign chlorophyll synthases in a cyanobacterial host. Synthesis of algal and plant chlorophyll synthases allowed deletion of the otherwise essential native cyanobacterial gene. Analysis of purified protein complexes shows that the interaction with YidC is maintained for both eukaryotic enzymes, indicating that a ChlG-YidC/Alb3 complex may be evolutionarily conserved in algae and plants.
References provided by Crossref.org
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- $a In the model cyanobacterium Synechocystis sp. PCC 6803, the terminal enzyme of chlorophyll biosynthesis, chlorophyll synthase (ChlG), forms a complex with high light-inducible proteins, the photosystem II assembly factor Ycf39 and the YidC/Alb3/OxaI membrane insertase, co-ordinating chlorophyll delivery with cotranslational insertion of nascent photosystem polypeptides into the membrane. To gain insight into the ubiquity of this assembly complex in higher photosynthetic organisms, we produced functional foreign chlorophyll synthases in a cyanobacterial host. Synthesis of algal and plant chlorophyll synthases allowed deletion of the otherwise essential native cyanobacterial gene. Analysis of purified protein complexes shows that the interaction with YidC is maintained for both eukaryotic enzymes, indicating that a ChlG-YidC/Alb3 complex may be evolutionarily conserved in algae and plants.
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