• Something wrong with this record ?

Acinetobacter cumulans sp. nov., isolated from hospital sewage and capable of acquisition of multiple antibiotic resistance genes

J. Qin, M. Maixnerová, M. Nemec, Y. Feng, X. Zhang, A. Nemec, Z. Zong,

. 2019 ; 42 (3) : 319-325. [pub] 20190215

Language English Country Germany

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc19034752

We studied the taxonomic position of six phenetically related strains of the genus Acinetobacter, which were recovered from hospital sewage in China and showed different patterns of resistance to clinically important antibiotics. Whole-genome sequencing of these strains and genus-wide phylogeny reconstruction based on a set of 107 Acinetobacter core genes indicated that they formed a separate and internally cohesive clade within the genus. The average nucleotide identity based on BLAST and digital DNA-DNA hybridization values between the six new genomes were 97.25-98.67% and 79.2-89.3%, respectively, whereas those between them and the genomes of the known species were ≤78.57% and ≤28.5%, respectively. The distinctness of the strains at the species level was also supported by the results of the cluster analysis of the whole-cell protein fingerprints generated by MALDI-TOF MS. Moreover, the strains displayed a catabolically unique profile and could be differentiated from the phylogenetically closest species at least by their inability to grow on d,l-lactate. A total of 18 different genes were found in the six genome sequences which encode resistance to seven classes of antimicrobial agents, including clinically important carbapenems, oxyimino-cephalosporins, or aminoglycosides. These genes occurred in five different combinations, with three to 10 different genes per strain. We conclude that the six strains represent a novel Acinetobacter species, for which we propose the name Acinetobacter cumulans sp. nov. to reflect its ability to acquire and cumulate diverse resistance determinants. The type strain is WCHAc060092T (ANC 5797T=CCTCC AB 2018119T=GDMCC 1.1380T=KCTC 62576T).

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19034752
003      
CZ-PrNML
005      
20191015115730.0
007      
ta
008      
191007s2019 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.syapm.2019.02.001 $2 doi
035    __
$a (PubMed)30808586
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Qin, Jiayuan $u Center of Infectious Diseases, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, Sichuan, China; Division of Infectious Diseases, State Key Laboratory of Biotherapy, Guoxuexiang 37, Chengdu 610041, Sichuan, China; Center for Pathogen Research, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, Sichuan, China.
245    10
$a Acinetobacter cumulans sp. nov., isolated from hospital sewage and capable of acquisition of multiple antibiotic resistance genes / $c J. Qin, M. Maixnerová, M. Nemec, Y. Feng, X. Zhang, A. Nemec, Z. Zong,
520    9_
$a We studied the taxonomic position of six phenetically related strains of the genus Acinetobacter, which were recovered from hospital sewage in China and showed different patterns of resistance to clinically important antibiotics. Whole-genome sequencing of these strains and genus-wide phylogeny reconstruction based on a set of 107 Acinetobacter core genes indicated that they formed a separate and internally cohesive clade within the genus. The average nucleotide identity based on BLAST and digital DNA-DNA hybridization values between the six new genomes were 97.25-98.67% and 79.2-89.3%, respectively, whereas those between them and the genomes of the known species were ≤78.57% and ≤28.5%, respectively. The distinctness of the strains at the species level was also supported by the results of the cluster analysis of the whole-cell protein fingerprints generated by MALDI-TOF MS. Moreover, the strains displayed a catabolically unique profile and could be differentiated from the phylogenetically closest species at least by their inability to grow on d,l-lactate. A total of 18 different genes were found in the six genome sequences which encode resistance to seven classes of antimicrobial agents, including clinically important carbapenems, oxyimino-cephalosporins, or aminoglycosides. These genes occurred in five different combinations, with three to 10 different genes per strain. We conclude that the six strains represent a novel Acinetobacter species, for which we propose the name Acinetobacter cumulans sp. nov. to reflect its ability to acquire and cumulate diverse resistance determinants. The type strain is WCHAc060092T (ANC 5797T=CCTCC AB 2018119T=GDMCC 1.1380T=KCTC 62576T).
650    _2
$a Acinetobacter $x chemie $x klasifikace $x účinky léků $x genetika $7 D000150
650    _2
$a antibakteriální látky $x farmakologie $7 D000900
650    _2
$a DNA bakterií $x genetika $7 D004269
650    _2
$a mnohočetná bakteriální léková rezistence $x genetika $7 D024901
650    12
$a bakteriální geny $7 D005798
650    _2
$a genom bakteriální $x genetika $7 D016680
650    12
$a nemocnice $7 D006761
650    _2
$a hybridizace nukleových kyselin $7 D009693
650    _2
$a peptidové mapování $7 D010449
650    _2
$a fylogeneze $7 D010802
650    _2
$a RNA ribozomální 16S $x genetika $7 D012336
650    _2
$a sekvenční analýza DNA $7 D017422
650    _2
$a odpadní vody $x mikrobiologie $7 D012722
651    _2
$a Čína $7 D002681
655    _2
$a časopisecké články $7 D016428
700    1_
$a Maixnerová, Martina $u Laboratory of Bacterial Genetics, Centre for Epidemiology and Microbiology, National Institute of Public Health, Šrobárova 48, 100 42 Prague 10, Czech Republic.
700    1_
$a Nemec, Matěj $u Laboratory of Bacterial Genetics, Centre for Epidemiology and Microbiology, National Institute of Public Health, Šrobárova 48, 100 42 Prague 10, Czech Republic.
700    1_
$a Feng, Yu $u Center of Infectious Diseases, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, Sichuan, China; Division of Infectious Diseases, State Key Laboratory of Biotherapy, Guoxuexiang 37, Chengdu 610041, Sichuan, China; Center for Pathogen Research, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, Sichuan, China.
700    1_
$a Zhang, Xinzhuo $u School of International Education, Southwest Medical University, No. 1 Xianglin Road, Luzhou 646000, Sichuan, China; Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, No. 1 Xianglin Road, Luzhou 646000, Sichuan, China.
700    1_
$a Nemec, Alexandr $u Laboratory of Bacterial Genetics, Centre for Epidemiology and Microbiology, National Institute of Public Health, Šrobárova 48, 100 42 Prague 10, Czech Republic; Department of Laboratory Medicine, Third Faculty of Medicine, Charles University, Šrobárova 50, 100 34 Prague 10, Czech Republic. Electronic address: alexandr.nemec@szu.cz.
700    1_
$a Zong, Zhiyong $u Center of Infectious Diseases, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, Sichuan, China; Division of Infectious Diseases, State Key Laboratory of Biotherapy, Guoxuexiang 37, Chengdu 610041, Sichuan, China; Center for Pathogen Research, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, Sichuan, China. Electronic address: zongzhiy@scu.edu.cn.
773    0_
$w MED00004831 $t Systematic and applied microbiology $x 1618-0984 $g Roč. 42, č. 3 (2019), s. 319-325
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30808586 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20191015120155 $b ABA008
999    __
$a ok $b bmc $g 1451412 $s 1073302
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 42 $c 3 $d 319-325 $e 20190215 $i 1618-0984 $m Systematic and applied microbiology $n Syst Appl Microbiol $x MED00004831
LZP    __
$a Pubmed-20191007

Find record

Citation metrics

Archiving options