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Low-molecular-weight paramagnetic 19F contrast agents for fluorine magnetic resonance imaging
V. Herynek, M. Martinisková, Y. Bobrova, A. Gálisová, J. Kotek, P. Hermann, F. Koucký, D. Jirák, M. Hájek,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
Grantová podpora
P205-16-03156S
Grantová Agentura České Republiky
Inter-COST, no. LTC17067, in the frame of CA15209 COST Action
Ministerstvo Školství, Mládeže a Tělovýchovy
Czech-BioImaging RI project LM2015062
Ministerstvo Školství, Mládeže a Tělovýchovy
CZ-DRO, IN 00023001
Ministerstvo Zdravotnictví Ceské Republiky
- MeSH
- chelátory chemie MeSH
- fluor chemie MeSH
- ionty MeSH
- kontrastní látky chemie MeSH
- krysa rodu rattus MeSH
- lanthanoidy chemie MeSH
- ligandy MeSH
- magnetismus MeSH
- molekulová hmotnost MeSH
- oxazoly chemie MeSH
- pyrimidinony chemie MeSH
- zobrazování fluorovou magnetickou rezonancí * MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVE: 19F MRI requires biocompatible and non-toxic soluble contrast agents with high fluorine content and with suitable 19F relaxation times. Probes based on a DOTP chelate with 12 magnetically equivalent fluorine atoms (DOTP-tfe) and a lanthanide(III) ion shortening the relaxation times were prepared and tested. METHODS: Complexes of DOTP-tfe with trivalent paramagnetic Ce, Dy, Ho, Tm, and Yb ions were synthetized and characterized. 19F relaxation times were determined and compared to those of the La complex and of the empty ligand. In vitro and in vivo 19F MRI was performed at 4.7 T. RESULTS: 19F relaxation times strongly depended on the chelated lanthanide(III) ion. T1 ranged from 6.5 to 287 ms, T2 from 3.9 to 124.4 ms, and T2* from 1.1 to 3.1 ms. All complexes in combination with optimized sequences provided sufficient signal in vitro under conditions mimicking experiments in vivo (concentrations 1.25 mM, 15-min scanning time). As a proof of concept, two contrast agents were injected into the rat muscle; 19F MRI in vivo confirmed the in vivo applicability of the probe. CONCLUSION: DOTP-based 19F probes showed suitable properties for in vitro and in vivo visualization and biological applications. The lanthanide(III) ions enabled us to shorten the relaxation times and to trim the probes according to the actual needs. Similar to the clinically approved Gd3+ chelates, this customized probe design ensures consistent biochemical properties and similar safety profiles.
Citace poskytuje Crossref.org
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- $a Herynek, Vít $u Center for Advanced Preclinical Imaging, First Faculty of Medicine, Charles University, Salmovská 3, Prague, Czech Republic. vit.herynek@lf1.cuni.cz. MR-Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, Prague, Czech Republic. vit.herynek@lf1.cuni.cz.
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- $a Low-molecular-weight paramagnetic 19F contrast agents for fluorine magnetic resonance imaging / $c V. Herynek, M. Martinisková, Y. Bobrova, A. Gálisová, J. Kotek, P. Hermann, F. Koucký, D. Jirák, M. Hájek,
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- $a OBJECTIVE: 19F MRI requires biocompatible and non-toxic soluble contrast agents with high fluorine content and with suitable 19F relaxation times. Probes based on a DOTP chelate with 12 magnetically equivalent fluorine atoms (DOTP-tfe) and a lanthanide(III) ion shortening the relaxation times were prepared and tested. METHODS: Complexes of DOTP-tfe with trivalent paramagnetic Ce, Dy, Ho, Tm, and Yb ions were synthetized and characterized. 19F relaxation times were determined and compared to those of the La complex and of the empty ligand. In vitro and in vivo 19F MRI was performed at 4.7 T. RESULTS: 19F relaxation times strongly depended on the chelated lanthanide(III) ion. T1 ranged from 6.5 to 287 ms, T2 from 3.9 to 124.4 ms, and T2* from 1.1 to 3.1 ms. All complexes in combination with optimized sequences provided sufficient signal in vitro under conditions mimicking experiments in vivo (concentrations 1.25 mM, 15-min scanning time). As a proof of concept, two contrast agents were injected into the rat muscle; 19F MRI in vivo confirmed the in vivo applicability of the probe. CONCLUSION: DOTP-based 19F probes showed suitable properties for in vitro and in vivo visualization and biological applications. The lanthanide(III) ions enabled us to shorten the relaxation times and to trim the probes according to the actual needs. Similar to the clinically approved Gd3+ chelates, this customized probe design ensures consistent biochemical properties and similar safety profiles.
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