• Something wrong with this record ?

Comparison of biofilm formation by Staphylococcus aureus and Staphylococcus epidermidis strains isolated from sheep milk using three diagnostic methods

M. Vasil', Z. Farkasova, J. Elecko, J. Illek, F. Zigo,

. 2017 ; 20 (4) : 795-801. [pub] -

Language English Country Germany

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc19035314

In this study we investigated 24 strains of Staphylococcus aureus and 33 strains of Staphylococcus epidermidis isolated from milk of sheep with clinical mastitis, for their ability to form biofilms. Three methods for the determination of a biofilm were used. When evaluating the growth on Congo Red agar (CRA), 79.2% S. aureus strains and 72.7% S. epidermidis strains were positive for biofilm formation. The quantitative method of biofilm detection on a Microtitre Plate (MTP) revealed positive results for 75.0% of S. aureus samples and 75.8% for S. epidermidis samples. Using PCR method for determination of the presence of genes that affect formation of biofilms, the most frequently determined genes were eno in both S. aureus (18/24; 75.0%) and S. epidermidis strains (20/33; 60.6%). The genes icaAB and ebpS were detected in both S. aureus and S. epidermidis strains, and similarity between these strains was 12.5% - 15.1% and 4.2% - 6.0%, respectively. The bap was recorded only in S. epidermidis (3.0%). Statistical comparison of the level of biofilm formation was performed using Chi square test. There were no statistically significant differences in the amount of biofilm formation between two methods for detection of biofilm CRA and MTP (p>0.05). Comparison of all six monitored parameters showed no dependence of characteristics of the tested strains S. aureus and S. epidermidis at significance level α = 0.05. Biofilm formation by the bacteria isolated from 57 cases of clinical mastitis in sheep was confirmed. Sensitivity and specificity of the CRA method for S. aureus were 94.44% and 66.66%, respectively, and for S. epidermidis 92.0% and 87.5%, respectively. Both CRA and MTP methods can be recommended for the detection of biofilm production by S. aureus and S. epidermidis strains isolated from milk of sheep with clinical mastitis.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19035314
003      
CZ-PrNML
005      
20191015102816.0
007      
ta
008      
191007s2017 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1515/pjvs-2017-0100 $2 doi
035    __
$a (PubMed)29611650
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Vasil', M $u Institute of Animal Breeding, Department of Nutrition, Dietetics and Animal Breeding, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 84 Kosice, the Slovak Republic.
245    10
$a Comparison of biofilm formation by Staphylococcus aureus and Staphylococcus epidermidis strains isolated from sheep milk using three diagnostic methods / $c M. Vasil', Z. Farkasova, J. Elecko, J. Illek, F. Zigo,
520    9_
$a In this study we investigated 24 strains of Staphylococcus aureus and 33 strains of Staphylococcus epidermidis isolated from milk of sheep with clinical mastitis, for their ability to form biofilms. Three methods for the determination of a biofilm were used. When evaluating the growth on Congo Red agar (CRA), 79.2% S. aureus strains and 72.7% S. epidermidis strains were positive for biofilm formation. The quantitative method of biofilm detection on a Microtitre Plate (MTP) revealed positive results for 75.0% of S. aureus samples and 75.8% for S. epidermidis samples. Using PCR method for determination of the presence of genes that affect formation of biofilms, the most frequently determined genes were eno in both S. aureus (18/24; 75.0%) and S. epidermidis strains (20/33; 60.6%). The genes icaAB and ebpS were detected in both S. aureus and S. epidermidis strains, and similarity between these strains was 12.5% - 15.1% and 4.2% - 6.0%, respectively. The bap was recorded only in S. epidermidis (3.0%). Statistical comparison of the level of biofilm formation was performed using Chi square test. There were no statistically significant differences in the amount of biofilm formation between two methods for detection of biofilm CRA and MTP (p>0.05). Comparison of all six monitored parameters showed no dependence of characteristics of the tested strains S. aureus and S. epidermidis at significance level α = 0.05. Biofilm formation by the bacteria isolated from 57 cases of clinical mastitis in sheep was confirmed. Sensitivity and specificity of the CRA method for S. aureus were 94.44% and 66.66%, respectively, and for S. epidermidis 92.0% and 87.5%, respectively. Both CRA and MTP methods can be recommended for the detection of biofilm production by S. aureus and S. epidermidis strains isolated from milk of sheep with clinical mastitis.
650    _2
$a zvířata $7 D000818
650    _2
$a bakteriologické techniky $7 D001431
650    _2
$a biofilmy $x růst a vývoj $7 D018441
650    _2
$a DNA bakterií $x izolace a purifikace $7 D004269
650    _2
$a mléko $x mikrobiologie $7 D008892
650    _2
$a senzitivita a specificita $7 D012680
650    _2
$a ovce $x mikrobiologie $7 D012756
650    _2
$a Staphylococcus aureus $x fyziologie $7 D013211
650    _2
$a Staphylococcus epidermidis $x fyziologie $7 D013212
655    _2
$a časopisecké články $7 D016428
700    1_
$a Farkasova, Z $u Institute of Animal Breeding, Department of Nutrition, Dietetics and Animal Breeding, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 84 Kosice, the Slovak Republic.
700    1_
$a Elecko, J $u Institute of Animal Breeding, Department of Nutrition, Dietetics and Animal Breeding, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 84 Kosice, the Slovak Republic.
700    1_
$a Illek, J $u Clinical Laboratory for Large Animals, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, the Czech Republic.
700    1_
$a Zigo, F $u Institute of Animal Breeding, Department of Nutrition, Dietetics and Animal Breeding, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 84 Kosice, the Slovak Republic.
773    0_
$w MED00181063 $t Polish journal of veterinary sciences $x 1505-1773 $g Roč. 20, č. 4 (2017), s. 795-801
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29611650 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20191015103241 $b ABA008
999    __
$a ok $b bmc $g 1451974 $s 1073864
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 20 $c 4 $d 795-801 $e - $i 1505-1773 $m Polish journal of veterinary sciences $n Pol J Vet Sci $x MED00181063
LZP    __
$a Pubmed-20191007
Back

Find record

Citation metrics

Archiving options