-
Je něco špatně v tomto záznamu ?
Long-Term Development of Embryonic Cerebellar Grafts in Two Strains of Lurcher Mice
J. Cendelin, Z. Purkartova, J. Kubik, E. Ulbricht, F. Tichanek, Y. Kolinko,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
716217
Grantová Agentura, Univerzita Karlova
National Sustainability Program I (NPU I) Nr. LO1503
Ministry of Education Youth and Sports of the Czech Republic
Q39
Univerzita Karlova v Praze
260 394
Univerzita Karlova v Praze
NLK
ProQuest Central
od 2002-03-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2002-01-01 do Před 1 rokem
Nursing & Allied Health Database (ProQuest)
od 2002-03-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2002-03-01 do Před 1 rokem
- MeSH
- druhová specificita MeSH
- longitudinální studie MeSH
- metoda rotující tyčky MeSH
- modely nemocí na zvířatech MeSH
- motorické dovednosti MeSH
- mozeček embryologie patologie transplantace MeSH
- myši - mutanty neurologické MeSH
- myši inbrední C3H MeSH
- myši inbrední CBA MeSH
- myši transgenní MeSH
- nemoci mozečku patologie patofyziologie terapie MeSH
- neurodegenerativní nemoci patologie patofyziologie terapie MeSH
- přežívání štěpu * fyziologie MeSH
- transplantace mozkové tkáně * MeSH
- zelené fluorescenční proteiny genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
For many degenerative cerebellar diseases, currently, no effective treatment that would substantially restore cerebellar functions is available. Neurotransplantation could be a promising therapy for such cases. Nevertheless, there are still severe limitations for routine clinical use. The aim of the work was to assess volume and morphology and functional impact on motor skills of an embryonic cerebellar graft injected in the form of cell suspension in Lurcher mutant and wild-type mice of the B6CBA and C3H strains after a 6-month survival period. The grafts survived in the majority of the mice. In both B6CBA and C3H Lurcher mice, most of the grafts were strictly delimited with no tendency to invade the host cerebellum, while in wild-type mice, graft-derived Purkinje cells colonized the host's cerebellum. In C3H Lurcher mice, but not in B6CBA Lurchers, the grafts had smaller volume than in their wild-type counterparts. C3H wild-type mice had significantly larger grafts than B6CBA wild-type mice. No positive effect of the transplantation on performance in the rotarod test was observed. The findings suggest that the niche of the Lurcher mutant cerebellum has a negative impact on integration of grafted cells. This factor seems to be limiting for specific functional effects of the transplantation therapy in this mouse model of cerebellar degeneration.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19035391
- 003
- CZ-PrNML
- 005
- 20250819110931.0
- 007
- ta
- 008
- 191007s2018 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12311-018-0928-3 $2 doi
- 035 __
- $a (PubMed)29450804
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Cendelin, Jan $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00, Plzen, Czech Republic. Jan.Cendelin@lfp.cuni.cz. Laboratory of Neurodegenerative Disorders, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00, Plzen, Czech Republic. Jan.Cendelin@lfp.cuni.cz.
- 245 10
- $a Long-Term Development of Embryonic Cerebellar Grafts in Two Strains of Lurcher Mice / $c J. Cendelin, Z. Purkartova, J. Kubik, E. Ulbricht, F. Tichanek, Y. Kolinko,
- 520 9_
- $a For many degenerative cerebellar diseases, currently, no effective treatment that would substantially restore cerebellar functions is available. Neurotransplantation could be a promising therapy for such cases. Nevertheless, there are still severe limitations for routine clinical use. The aim of the work was to assess volume and morphology and functional impact on motor skills of an embryonic cerebellar graft injected in the form of cell suspension in Lurcher mutant and wild-type mice of the B6CBA and C3H strains after a 6-month survival period. The grafts survived in the majority of the mice. In both B6CBA and C3H Lurcher mice, most of the grafts were strictly delimited with no tendency to invade the host cerebellum, while in wild-type mice, graft-derived Purkinje cells colonized the host's cerebellum. In C3H Lurcher mice, but not in B6CBA Lurchers, the grafts had smaller volume than in their wild-type counterparts. C3H wild-type mice had significantly larger grafts than B6CBA wild-type mice. No positive effect of the transplantation on performance in the rotarod test was observed. The findings suggest that the niche of the Lurcher mutant cerebellum has a negative impact on integration of grafted cells. This factor seems to be limiting for specific functional effects of the transplantation therapy in this mouse model of cerebellar degeneration.
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a transplantace mozkové tkáně $7 D016380
- 650 _2
- $a nemoci mozečku $x patologie $x patofyziologie $x terapie $7 D002526
- 650 _2
- $a mozeček $x embryologie $x patologie $x transplantace $7 D002531
- 650 _2
- $a modely nemocí na zvířatech $7 D004195
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 12
- $a přežívání štěpu $x fyziologie $7 D006085
- 650 _2
- $a zelené fluorescenční proteiny $x genetika $x metabolismus $7 D049452
- 650 _2
- $a longitudinální studie $7 D008137
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a myši inbrední C3H $7 D008809
- 650 _2
- $a myši inbrední CBA $7 D008808
- 650 _2
- $a myši - mutanty neurologické $7 D008818
- 650 _2
- $a myši transgenní $7 D008822
- 650 _2
- $a motorické dovednosti $7 D009048
- 650 _2
- $a neurodegenerativní nemoci $x patologie $x patofyziologie $x terapie $7 D019636
- 650 _2
- $a metoda rotující tyčky $7 D045442
- 650 _2
- $a druhová specificita $7 D013045
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Purkartová, Zdeňka, $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00, Plzen, Czech Republic. $d 1974- $7 xx0326689
- 700 1_
- $a Kubik, Jakub $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00, Plzen, Czech Republic.
- 700 1_
- $a Ulbricht, Erik $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00, Plzen, Czech Republic.
- 700 1_
- $a Tichánek, Filip $u Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00, Plzen, Czech Republic. Laboratory of Neurodegenerative Disorders, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 1655/76, 323 00, Plzen, Czech Republic. $7 xx0334827
- 700 1_
- $a Kolinko, Yaroslav $u Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66, Plzen, Czech Republic. Laboratory of Quantitative Histology, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66, Plzen, Czech Republic.
- 773 0_
- $w MED00007845 $t Cerebellum $x 1473-4230 $g Roč. 17, č. 4 (2018), s. 428-437
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/29450804 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20191007 $b ABA008
- 991 __
- $a 20250819110914 $b ABA008
- 999 __
- $a ok $b bmc $g 1452051 $s 1073941
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2018 $b 17 $c 4 $d 428-437 $e - $i 1473-4230 $m Cerebellum $n Cerebellum $x MED00007845
- GRA __
- $a 716217 $p Grantová Agentura, Univerzita Karlova
- GRA __
- $a National Sustainability Program I (NPU I) Nr. LO1503 $p Ministry of Education Youth and Sports of the Czech Republic
- GRA __
- $a Q39 $p Univerzita Karlova v Praze
- GRA __
- $a 260 394 $p Univerzita Karlova v Praze
- LZP __
- $a Pubmed-20191007
