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Association of kidney fibrosis with urinary peptides: a path towards non-invasive liquid biopsies?
P. Magalhães, M. Pejchinovski, K. Markoska, M. Banasik, M. Klinger, D. Švec-Billá, I. Rychlík, M. Rroji, A. Restivo, G. Capasso, F. Bob, A. Schiller, A. Ortiz, MV. Perez-Gomez, P. Cannata, MD. Sanchez-Niño, R. Naumovic, V. Brkovic, M....
Language English Country England, Great Britain
Document type Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't
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- MeSH
- Renal Insufficiency, Chronic pathology urine MeSH
- Adult MeSH
- Electrophoresis, Capillary MeSH
- Fibrosis pathology urine MeSH
- Mass Spectrometry MeSH
- Collagen urine MeSH
- Kidney pathology MeSH
- Middle Aged MeSH
- Humans MeSH
- Peptides urine MeSH
- Liquid Biopsy methods MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Multicenter Study MeSH
- Research Support, Non-U.S. Gov't MeSH
Chronic kidney disease (CKD) is a prevalent cause of morbidity and mortality worldwide. A hallmark of CKD progression is renal fibrosis characterized by excessive accumulation of extracellular matrix (ECM) proteins. In this study, we aimed to investigate the correlation of the urinary proteome classifier CKD273 and individual urinary peptides with the degree of fibrosis. In total, 42 kidney biopsies and urine samples were examined. The percentage of fibrosis per total tissue area was assessed in Masson trichrome stained kidney tissues. The urinary proteome was analysed by capillary electrophoresis coupled to mass spectrometry. CKD273 displayed a significant and positive correlation with the degree of fibrosis (Rho = 0.430, P = 0.0044), while the routinely used parameters (glomerular filtration rate, urine albumin-to-creatinine ratio and urine protein-to-creatinine ratio) did not (Rho = -0.222; -0.137; -0.070 and P = 0.16; 0.39; 0.66, respectively). We identified seven fibrosis-associated peptides displaying a significant and negative correlation with the degree of fibrosis. All peptides were collagen fragments, suggesting that these may be causally related to the observed accumulation of ECM in the kidneys. CKD273 and specific peptides are significantly associated with kidney fibrosis; such an association could not be detected by other biomarkers for CKD. These non-invasive fibrosis-related biomarkers can potentially be implemented in future trials.
1st Department of Medicine 3rd Faculty of Medicine Charles University Prague Czech Republic
Biotechnology Division Biomedical Research Foundation Academy of Athens Athens Greece
Department of Nephrology and Transplantation Medicine Wroclaw Medical University Wroclaw Poland
Department of Nephrology Medical Faculty University of Skopje Skopje Macedonia
Department of Nephrology University Hospital Center Mother Teresa Tirana Albania
Department of Nephrology University of Campania Luigi Vanvitelli Naples Italy
Department of Pediatric Nephrology Hannover Medical School Hannover Germany
IIS Fundacion Jimenez Diaz UAM Madrid Spain
Institute of Cardiovascular and Medical Sciences University of Glasgow Glasgow UK
Macedonian Academy of Sciences and Arts Skopje Macedonia
References provided by Crossref.org
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