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Genome sequencing reveals metabolic and cellular interdependence in an amoeba-kinetoplastid symbiosis
G. Tanifuji, U. Cenci, D. Moog, S. Dean, T. Nakayama, V. David, I. Fiala, BA. Curtis, SJ. Sibbald, NT. Onodera, M. Colp, P. Flegontov, J. Johnson-MacKinnon, M. McPhee, Y. Inagaki, T. Hashimoto, S. Kelly, K. Gull, J. Lukeš, JM. Archibald,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
Wellcome Trust - United Kingdom
NLK
Directory of Open Access Journals
od 2011
Free Medical Journals
od 2011
Nature Open Access
od 2011-12-01
PubMed Central
od 2011
Europe PubMed Central
od 2011
ProQuest Central
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Health & Medicine (ProQuest)
od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2011
- MeSH
- Amoebozoa genetika růst a vývoj metabolismus MeSH
- genom protozoální MeSH
- Kinetoplastida genetika růst a vývoj metabolismus MeSH
- sekvenční analýza DNA MeSH
- symbióza * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Endosymbiotic relationships between eukaryotic and prokaryotic cells are common in nature. Endosymbioses between two eukaryotes are also known; cyanobacterium-derived plastids have spread horizontally when one eukaryote assimilated another. A unique instance of a non-photosynthetic, eukaryotic endosymbiont involves members of the genus Paramoeba, amoebozoans that infect marine animals such as farmed fish and sea urchins. Paramoeba species harbor endosymbionts belonging to the Kinetoplastea, a diverse group of flagellate protists including some that cause devastating diseases. To elucidate the nature of this eukaryote-eukaryote association, we sequenced the genomes and transcriptomes of Paramoeba pemaquidensis and its endosymbiont Perkinsela sp. The endosymbiont nuclear genome is ~9.5 Mbp in size, the smallest of a kinetoplastid thus far discovered. Genomic analyses show that Perkinsela sp. has lost the ability to make a flagellum but retains hallmark features of kinetoplastid biology, including polycistronic transcription, trans-splicing, and a glycosome-like organelle. Mosaic biochemical pathways suggest extensive 'cross-talk' between the two organisms, and electron microscopy shows that the endosymbiont ingests amoeba cytoplasm, a novel form of endosymbiont-host communication. Our data reveal the cell biological and biochemical basis of the obligate relationship between Perkinsela sp. and its amoeba host, and provide a foundation for understanding pathogenicity determinants in economically important Paramoeba.
Department of Plant Sciences University of Oxford Oxford United Kingdom
Graduate School of Life and Environmental Sciences University of Tsukuba Tsukuba Japan
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
Sir William Dunn School of Pathology University of Oxford Oxford United Kingdom
Citace poskytuje Crossref.org
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- $a Tanifuji, Goro $u Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada. Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada. Department of Zoology, National Museum of Nature and Science, Tsukuba, Japan.
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- $a Endosymbiotic relationships between eukaryotic and prokaryotic cells are common in nature. Endosymbioses between two eukaryotes are also known; cyanobacterium-derived plastids have spread horizontally when one eukaryote assimilated another. A unique instance of a non-photosynthetic, eukaryotic endosymbiont involves members of the genus Paramoeba, amoebozoans that infect marine animals such as farmed fish and sea urchins. Paramoeba species harbor endosymbionts belonging to the Kinetoplastea, a diverse group of flagellate protists including some that cause devastating diseases. To elucidate the nature of this eukaryote-eukaryote association, we sequenced the genomes and transcriptomes of Paramoeba pemaquidensis and its endosymbiont Perkinsela sp. The endosymbiont nuclear genome is ~9.5 Mbp in size, the smallest of a kinetoplastid thus far discovered. Genomic analyses show that Perkinsela sp. has lost the ability to make a flagellum but retains hallmark features of kinetoplastid biology, including polycistronic transcription, trans-splicing, and a glycosome-like organelle. Mosaic biochemical pathways suggest extensive 'cross-talk' between the two organisms, and electron microscopy shows that the endosymbiont ingests amoeba cytoplasm, a novel form of endosymbiont-host communication. Our data reveal the cell biological and biochemical basis of the obligate relationship between Perkinsela sp. and its amoeba host, and provide a foundation for understanding pathogenicity determinants in economically important Paramoeba.
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- $a Dean, Samuel $u Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
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- $a Nakayama, Takuro $u Center for Computational Sciences, University of Tsukuba, Tsukuba, Japan. Graduate School of Life Sciences, Tohoku University, Tohoku, Japan.
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