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Melanoma-related changes in skin microbiome
J. Mrázek, C. Mekadim, P. Kučerová, R. Švejstil, H. Salmonová, J. Vlasáková, R. Tarasová, J. Čížková, M. Červinková,
Language English Country United States
Document type Journal Article
Grant support
CIGA 20162019
Fakultu Agrobiologie, Potravinových a Prírodních Zdroju, Ceská Zemedelská Univerzita v Praze
CIGA 20162001
Fakultu Agrobiologie, Potravinových a Prírodních Zdroju, Ceská Zemedelská Univerzita v Praze
CZ.02.1.01/0.0/0.0/15_003/0000460
Ministerstvo Školství, Mládeže a Telovýchovy
LO1609
Ministerstvo Školství, Mládeže a Telovýchovy
- MeSH
- Bacteria classification isolation & purification MeSH
- DNA, Bacterial genetics MeSH
- DNA Primers MeSH
- Fusobacterium genetics isolation & purification MeSH
- Genetic Variation MeSH
- Skin microbiology MeSH
- Melanoma microbiology pathology MeSH
- Microbiota * MeSH
- Swine, Miniature MeSH
- Disease Models, Animal MeSH
- Swine MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
Melanoma is the least common form of skin tumor, but it is potentially the most dangerous and responsible for the majority of skin cancer deaths. We suggest that the skin microbiome might be changed during the progression of melanoma. The aim of this study is to compare the composition of the skin microbiota between different locations (skin and melanoma) of a MeLiM (Melanoma-bearing Libechov Minipig) pig model (exophytic melanoma). Ninety samples were used for PCR-DGGE analysis with primers specifically targeting the V3 region of the 16S rRNA gene. The profiles were used for cluster analysis by UPGMA and principal coordinate analysis PCoA and also to calculate the diversity index (Simpson index of diversity). By comparing the obtained results, we found that both bacterial composition and diversity were significantly different between the skin and melanoma microbiomes. The abundances of Fusobacterium and Trueperella genera were significantly increased in melanoma samples, suggesting a strong relationship between melanoma development and skin microbiome changes.
References provided by Crossref.org
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- $a Melanoma is the least common form of skin tumor, but it is potentially the most dangerous and responsible for the majority of skin cancer deaths. We suggest that the skin microbiome might be changed during the progression of melanoma. The aim of this study is to compare the composition of the skin microbiota between different locations (skin and melanoma) of a MeLiM (Melanoma-bearing Libechov Minipig) pig model (exophytic melanoma). Ninety samples were used for PCR-DGGE analysis with primers specifically targeting the V3 region of the 16S rRNA gene. The profiles were used for cluster analysis by UPGMA and principal coordinate analysis PCoA and also to calculate the diversity index (Simpson index of diversity). By comparing the obtained results, we found that both bacterial composition and diversity were significantly different between the skin and melanoma microbiomes. The abundances of Fusobacterium and Trueperella genera were significantly increased in melanoma samples, suggesting a strong relationship between melanoma development and skin microbiome changes.
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- $a Tarasová, Renata $u Faculty of Biomedical Engineering, Czech Technical University in Prague, Nám. Sítná 3105, 272 01, Kladno, Czech Republic.
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