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Melanoma-related changes in skin microbiome
J. Mrázek, C. Mekadim, P. Kučerová, R. Švejstil, H. Salmonová, J. Vlasáková, R. Tarasová, J. Čížková, M. Červinková,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
CIGA 20162019
Fakultu Agrobiologie, Potravinových a Prírodních Zdroju, Ceská Zemedelská Univerzita v Praze
CIGA 20162001
Fakultu Agrobiologie, Potravinových a Prírodních Zdroju, Ceská Zemedelská Univerzita v Praze
CZ.02.1.01/0.0/0.0/15_003/0000460
Ministerstvo Školství, Mládeže a Telovýchovy
LO1609
Ministerstvo Školství, Mládeže a Telovýchovy
- MeSH
- Bacteria klasifikace izolace a purifikace MeSH
- DNA bakterií genetika MeSH
- DNA primery MeSH
- Fusobacterium genetika izolace a purifikace MeSH
- genetická variace MeSH
- kůže mikrobiologie MeSH
- melanom mikrobiologie patologie MeSH
- mikrobiota * MeSH
- miniaturní prasata MeSH
- modely nemocí na zvířatech MeSH
- prasata MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Melanoma is the least common form of skin tumor, but it is potentially the most dangerous and responsible for the majority of skin cancer deaths. We suggest that the skin microbiome might be changed during the progression of melanoma. The aim of this study is to compare the composition of the skin microbiota between different locations (skin and melanoma) of a MeLiM (Melanoma-bearing Libechov Minipig) pig model (exophytic melanoma). Ninety samples were used for PCR-DGGE analysis with primers specifically targeting the V3 region of the 16S rRNA gene. The profiles were used for cluster analysis by UPGMA and principal coordinate analysis PCoA and also to calculate the diversity index (Simpson index of diversity). By comparing the obtained results, we found that both bacterial composition and diversity were significantly different between the skin and melanoma microbiomes. The abundances of Fusobacterium and Trueperella genera were significantly increased in melanoma samples, suggesting a strong relationship between melanoma development and skin microbiome changes.
Citace poskytuje Crossref.org
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- $a Melanoma is the least common form of skin tumor, but it is potentially the most dangerous and responsible for the majority of skin cancer deaths. We suggest that the skin microbiome might be changed during the progression of melanoma. The aim of this study is to compare the composition of the skin microbiota between different locations (skin and melanoma) of a MeLiM (Melanoma-bearing Libechov Minipig) pig model (exophytic melanoma). Ninety samples were used for PCR-DGGE analysis with primers specifically targeting the V3 region of the 16S rRNA gene. The profiles were used for cluster analysis by UPGMA and principal coordinate analysis PCoA and also to calculate the diversity index (Simpson index of diversity). By comparing the obtained results, we found that both bacterial composition and diversity were significantly different between the skin and melanoma microbiomes. The abundances of Fusobacterium and Trueperella genera were significantly increased in melanoma samples, suggesting a strong relationship between melanoma development and skin microbiome changes.
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