Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Oral Microbiota Composition and Antimicrobial Antibody Response in Patients with Recurrent Aphthous Stomatitis

Z. Stehlikova, V. Tlaskal, N. Galanova, R. Roubalova, J. Kreisinger, J. Dvorak, P. Prochazkova, K. Kostovcikova, J. Bartova, M. Libanska, R. Cermakova, D. Schierova, A. Fassmann, P. Borilova Linhartova, S. Coufal, M. Kverka, L. Izakovicova-Holla,...

. 2019 ; 7 (12) : . [pub] 20191201

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc19043946

Grant support
15-29336A Ministry of Health of the Czech Republic
GAUK 1366217 Grant Agency of Charles University
GAUK 908217 Grant Agency of Charles University

Recurrent aphthous stomatitis (RAS) is the most common disease of the oral mucosa, and it has been recently associated with bacterial and fungal dysbiosis. To study this link further, we investigated microbial shifts during RAS manifestation at an ulcer site, in its surroundings, and at an unaffected site, compared with healed mucosa in RAS patients and healthy controls. We sampled microbes from five distinct sites in the oral cavity. The one site with the most pronounced differences in microbial alpha and beta diversity between RAS patients and healthy controls was the lower labial mucosa. Detailed analysis of this particular oral site revealed strict association of the genus Selenomonas with healed mucosa of RAS patients, whereas the class Clostridia and genera Lachnoanaerobaculum, Cardiobacterium, Leptotrichia, and Fusobacterium were associated with the presence of an active ulcer. Furthermore, active ulcers were dominated by Malassezia, which were negatively correlated with Streptococcus and Haemophilus and positively correlated with Porphyromonas species. In addition, RAS patients showed increased serum levels of IgG against Mogibacteriumtimidum compared with healthy controls. Our study demonstrates that the composition of bacteria and fungi colonizing healthy oral mucosa is changed in active RAS ulcers, and that this alteration persists to some extent even after the ulcer is healed.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19043946
003      
CZ-PrNML
005      
20250513114549.0
007      
ta
008      
200107s2019 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/microorganisms7120636 $2 doi
035    __
$a (PubMed)31805744
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Stehlikova, Zuzana $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic. First Faculty of Medicine, Charles University, 121 08 Prague, Czech Republic.
245    10
$a Oral Microbiota Composition and Antimicrobial Antibody Response in Patients with Recurrent Aphthous Stomatitis / $c Z. Stehlikova, V. Tlaskal, N. Galanova, R. Roubalova, J. Kreisinger, J. Dvorak, P. Prochazkova, K. Kostovcikova, J. Bartova, M. Libanska, R. Cermakova, D. Schierova, A. Fassmann, P. Borilova Linhartova, S. Coufal, M. Kverka, L. Izakovicova-Holla, J. Petanova, H. Tlaskalova-Hogenova, Z. Jiraskova Zakostelska,
520    9_
$a Recurrent aphthous stomatitis (RAS) is the most common disease of the oral mucosa, and it has been recently associated with bacterial and fungal dysbiosis. To study this link further, we investigated microbial shifts during RAS manifestation at an ulcer site, in its surroundings, and at an unaffected site, compared with healed mucosa in RAS patients and healthy controls. We sampled microbes from five distinct sites in the oral cavity. The one site with the most pronounced differences in microbial alpha and beta diversity between RAS patients and healthy controls was the lower labial mucosa. Detailed analysis of this particular oral site revealed strict association of the genus Selenomonas with healed mucosa of RAS patients, whereas the class Clostridia and genera Lachnoanaerobaculum, Cardiobacterium, Leptotrichia, and Fusobacterium were associated with the presence of an active ulcer. Furthermore, active ulcers were dominated by Malassezia, which were negatively correlated with Streptococcus and Haemophilus and positively correlated with Porphyromonas species. In addition, RAS patients showed increased serum levels of IgG against Mogibacteriumtimidum compared with healthy controls. Our study demonstrates that the composition of bacteria and fungi colonizing healthy oral mucosa is changed in active RAS ulcers, and that this alteration persists to some extent even after the ulcer is healed.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Tláskal, Vojtěch, $d 1991- $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic. Faculty of Science, Charles University, 128 00 Prague, Czech Republic. $7 mzk2013740878
700    1_
$a Galanova, Natalie $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic. Faculty of Science, Charles University, 128 00 Prague, Czech Republic.
700    1_
$a Roubalova, Radka $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic.
700    1_
$a Kreisinger, Jakub $u Department of Zoology, Faculty of Science, Charles University, 128 00 Prague, Czech Republic.
700    1_
$a Dvorak, Jiri $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic.
700    1_
$a Prochazkova, Petra $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic.
700    1_
$a Kostovcikova, Klara $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic.
700    1_
$a Bartova, Jirina $u Institute of Dental Medicine, Department of Oral Medicine, General University Hospital in Prague, 128 00 Prague, Czech Republic.
700    1_
$a Libanska, Marketa $u Institute of Dental Medicine, Department of Oral Medicine, General University Hospital in Prague, 128 00 Prague, Czech Republic.
700    1_
$a Cermakova, Radka $u Institute of Dental Medicine, Department of Oral Medicine, General University Hospital in Prague, 128 00 Prague, Czech Republic.
700    1_
$a Schierova, Dagmar $u Institute of Animal Physiology and Genetics of the CAS, v.v.i., 142 20 Prague, Czech Republic.
700    1_
$a Fassmann, Antonín, $d 1943-2025 $u Clinic of Dentistry, St. Anne's Faculty Hospital, 656 91, Brno, Czech Republic. Clinic of Dentistry, Faculty of Medicine, Masaryk University,625 00, Brno, Czech Republic. $7 xx0003678
700    1_
$a Borilova Linhartova, Petra $u Clinic of Dentistry, St. Anne's Faculty Hospital, 656 91, Brno, Czech Republic. Clinic of Dentistry, Faculty of Medicine, Masaryk University,625 00, Brno, Czech Republic.
700    1_
$a Coufal, Stepan $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic.
700    1_
$a Kverka, Miloslav $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic. Institute of Experimental Medicine of the CAS, v.v.i., 142 20 Prague, Czech Republic.
700    1_
$a Izakovicova-Holla, Lydie $u Clinic of Dentistry, St. Anne's Faculty Hospital, 656 91, Brno, Czech Republic. Clinic of Dentistry, Faculty of Medicine, Masaryk University,625 00, Brno, Czech Republic.
700    1_
$a Petanova, Jitka $u Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University and General University Hospital in Prague, 128 00 Prague, Czech Republic.
700    1_
$a Tlaskalova-Hogenova, Helena $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic. First Faculty of Medicine, Charles University, 121 08 Prague, Czech Republic.
700    1_
$a Jiraskova Zakostelska, Zuzana $u Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic.
773    0_
$w MED00198767 $t Microorganisms $x 2076-2607 $g Roč. 7, č. 12 (2019)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31805744 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200107 $b ABA008
991    __
$a 20250513114552 $b ABA008
999    __
$a ind $b bmc $g 1480535 $s 1082616
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 7 $c 12 $e 20191201 $i 2076-2607 $m Microorganisms $n Microorganisms $x MED00198767
GRA    __
$a 15-29336A $p Ministry of Health of the Czech Republic
GRA    __
$a GAUK 1366217 $p Grant Agency of Charles University
GRA    __
$a GAUK 908217 $p Grant Agency of Charles University
LZP    __
$a Pubmed-20200107
Back

Find record

Citation metrics

Archiving options