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Effect of AgNPs on the human reconstructed epidermis
J. Franková, J. Juráňová, V. Kamarád, B. Zálešák, J. Ulrichová
Jazyk angličtina Země Slovensko
Typ dokumentu časopisecké články
NLK
Free Medical Journals
od 2008
PubMed Central
od 2008 do 2019
Europe PubMed Central
od 2008 do 2018
ProQuest Central
od 2008-06-01 do 2019-01-31
Open Access Digital Library
od 2008-01-01
Open Access Digital Library
od 2008-01-01
Open Access Digital Library
od 2009-06-19
Nursing & Allied Health Database (ProQuest)
od 2008-06-01 do 2019-01-31
Health & Medicine (ProQuest)
od 2008-06-01 do 2019-01-31
Public Health Database (ProQuest)
od 2008-06-01 do 2019-01-31
Sciendo
od 2009-06-19
ROAD: Directory of Open Access Scholarly Resources
od 2008
PubMed
31762680
DOI
10.2478/intox-2018-0028
Knihovny.cz E-zdroje
- MeSH
- buněčné kultury MeSH
- epidermis * patologie účinky léků MeSH
- keratinocyty metabolismus MeSH
- kovové nanočástice * MeSH
- lidé MeSH
- stříbro farmakologie toxicita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Nanoparticles are utilized in a wide range of industries. The most studied silver nanoparticles (AgNPs) are used in medicine and also in several wound dressings due to their antimicrobial properties. The inflammatory response or potential morphological changes of skin cells after their application are not well known yet. In our study we used the model of human reconstructed epidermis (RHE), prepared in our laboratory, to evaluate whether the AgNPs penetrate through RHE, induce some morphological changes of keratinocytes or influence the production of pro-inflammatory cytokines (IL-6 and IL-8). After the application of three different concentrations (25 ppm, 2.5 ppm, 0.25 ppm) of AgNPs to of RHE for 24 hours we verified that AgNPs did not affect the production of pro-inflammatory cytokines (IL-6 and IL-8) and neither did they influence the expression of keratin K14 and loricrin. The morphology of the cells was likewise unchanged. Based on these results we conclude that AgNPs do not have any negative effect on the morphological changes and do not increase the production of pro-inflammatory cytokines.
Department of Histology and Embryology Faculty of Medicine and Dentistry Olomouc Czech Republic
Department of Plastic and Aesthetic Surgery University Hospital Olomouc Olomouc Czech Republic
Literatura
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