Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Novel FRET-Based Src Biosensor Reveals Mechanisms of Src Activation and Its Dynamics in Focal Adhesions

L. Koudelková, AC. Pataki, O. Tolde, V. Pavlik, M. Nobis, J. Gemperle, K. Anderson, J. Brábek, D. Rosel,

. 2019 ; 26 (2) : 255-268.e4. [pub] 20181213

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19045089

Src kinase plays an important role in a multitude of fundamental cellular processes and is often found deregulated in tumors. Active Src adopts an open conformation, whereas inactive Src is characterized by a very compact structure stabilized by inhibitory intramolecular interactions. Taking advantage of this spatial regulation, we constructed a fluorescence resonance energy transfer (FRET)-based Src biosensor and analyzed conformational changes of Src following Src activation and the spatiotemporal dynamics of Src activity in cells. We found that activatory mutations either in regulatory or kinase domains induce opening of the Src structure. Surprisingly, we discovered that Src inhibitors differ in their effect on the Src structure, some counterintuitively inducing an open conformation. Finally, we analyzed the dynamics of Src activity in focal adhesions by FRET imaging and found that Src is rapidly activated during focal adhesion assembly, and its activity remains steady and high throughout the life cycle of focal adhesion and decreases during focal adhesion disassembly.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19045089
003      
CZ-PrNML
005      
20220119145913.0
007      
ta
008      
200109s2019 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.chembiol.2018.10.024 $2 doi
035    __
$a (PubMed)30554912
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Koudelková, Lenka $u Charles University, Faculty of Science - BIOCEV, Department of Cell Biology, Vestec 252 50, Czech Republic.
245    10
$a Novel FRET-Based Src Biosensor Reveals Mechanisms of Src Activation and Its Dynamics in Focal Adhesions / $c L. Koudelková, AC. Pataki, O. Tolde, V. Pavlik, M. Nobis, J. Gemperle, K. Anderson, J. Brábek, D. Rosel,
520    9_
$a Src kinase plays an important role in a multitude of fundamental cellular processes and is often found deregulated in tumors. Active Src adopts an open conformation, whereas inactive Src is characterized by a very compact structure stabilized by inhibitory intramolecular interactions. Taking advantage of this spatial regulation, we constructed a fluorescence resonance energy transfer (FRET)-based Src biosensor and analyzed conformational changes of Src following Src activation and the spatiotemporal dynamics of Src activity in cells. We found that activatory mutations either in regulatory or kinase domains induce opening of the Src structure. Surprisingly, we discovered that Src inhibitors differ in their effect on the Src structure, some counterintuitively inducing an open conformation. Finally, we analyzed the dynamics of Src activity in focal adhesions by FRET imaging and found that Src is rapidly activated during focal adhesion assembly, and its activity remains steady and high throughout the life cycle of focal adhesion and decreases during focal adhesion disassembly.
650    _2
$a biosenzitivní techniky $x metody $7 D015374
650    _2
$a FRAP $7 D036681
650    _2
$a rezonanční přenos fluorescenční energie $7 D031541
650    _2
$a fokální adheze $x metabolismus $7 D022001
650    _2
$a HEK293 buňky $7 D057809
650    _2
$a lidé $7 D006801
650    _2
$a mutageneze $7 D016296
650    _2
$a skupina kinas odvozených od src-genu $x antagonisté a inhibitory $x genetika $x metabolismus $7 D019061
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Pataki, Andreea Csilla $u Charles University, Faculty of Science - BIOCEV, Department of Cell Biology, Vestec 252 50, Czech Republic.
700    1_
$a Tolde, Ondřej $u Charles University, Faculty of Science - BIOCEV, Department of Cell Biology, Vestec 252 50, Czech Republic.
700    1_
$a Pavlik, Vojtech $u Charles University, Faculty of Science - BIOCEV, Department of Cell Biology, Vestec 252 50, Czech Republic.
700    1_
$a Nobis, Max $u Cancer Research UK, Beatson Institute, Glasgow G611BD, UK.
700    1_
$a Gemperle, Jakub $u Charles University, Faculty of Science - BIOCEV, Department of Cell Biology, Vestec 252 50, Czech Republic.
700    1_
$a Anderson, Kurt $u Cancer Research UK, Beatson Institute, Glasgow G611BD, UK.
700    1_
$a Brábek, Jan, $u Charles University, Faculty of Science - BIOCEV, Department of Cell Biology, Vestec 252 50, Czech Republic. $d 1973- $7 xx0268678
700    1_
$a Rosel, Daniel $u Charles University, Faculty of Science - BIOCEV, Department of Cell Biology, Vestec 252 50, Czech Republic. Electronic address: rosel@natur.cuni.cz.
773    0_
$w MED00195160 $t Cell chemical biology $x 2451-9448 $g Roč. 26, č. 2 (2019), s. 255-268.e4
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30554912 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200109 $b ABA008
991    __
$a 20220119145910 $b ABA008
999    __
$a ok $b bmc $g 1483358 $s 1083762
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 26 $c 2 $d 255-268.e4 $e 20181213 $i 2451-9448 $m Cell chemical biology $n Cell Chem Biol $x MED00195160
LZP    __
$a Pubmed-20200109

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...