Fluorescence Recovery After Photobleaching [FRAP]

topical
25
Terms

Fluorescence Photobleaching Recovery
FRAP (Fluorescence Recovery After Photobleaching)

Persistent link   https://www.medvik.cz/link/D036681
Definition

A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).

DUI
D036681 MeSH Browser
CUI
M0410438
Previous indexing
Fluorescence (1976-2002)
History note
2003
Public note
2003

Allowable subheadings

CL
classification
EC
economics
ES
ethics
HI
history
IS
instrumentation
MT
methods 3
ST
standards
SN
statistics & numerical data 1
TD
trends
VE
veterinary

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E01 Diagnosis 1 790
E02 Therapeutics 2 575
E03 Anesthesia and Analgesia 168
E04 Surgical Procedures, Operative 4 389
E05 Investigative Techniques 209
E05.196 Chemistry Techniques, Analytical 1 182
E05.196.712 Photometry 381
E05.196.712.516 Luminescent Measurements 119
E05.196.712.516.600 Fluorometry 183
E05.196.712.516.600.240 Cytophotometry 27
E05.196.712.516.600.390 Fluorescence Polarization 48
E05.196.712.516.600.393 Fluorescence Recovery After Photobleaching 25
E05.196.712.516.600.410 Fluorophotometry 3
E05.196.712.516.600.543 Quantitative Light-Induced Fluorescence
E05.196.712.516.600.676 Spectrometry, Fluorescence 412
E06 Dentistry 1 670
E07 Equipment and Supplies 4 036

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