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Hmotnostní spektrometrie s indukčně vázaným plazmatem – analýza jediné buňky
[Inductively coupled plasma mass spectrometry - single-cell analysis]
Tomáš Pluháček,Vítězslav Maier
Jazyk čeština Země Česko
Typ dokumentu práce podpořená grantem
- MeSH
- analýza jednotlivých buněk metody MeSH
- hmotnostní spektrometrie * metody MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
The cell, as a fundamental structural, functional and biological unit, plays an essential role in living organisms. Analysis of the molecular/elemental composition of a single cell is a significant aspect of lipidomic, proteomic, metabolomic and metallomic studies aiming at understanding molecular processes in cells. Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful technique which can help to elucidate the bioeffects of trace metals and their species on cellular metabolism and cell behavior. Numerous ICP-MS-based methods have already been utilized to explore elemental/species profiles. These include, e.g. time-resolved ICP-MS, electrothermal vaporization ICP-MS, laser ablation ICP-MS, chip-based microextraction techniques, HPLC/CE-ICP-MS, elemental tagging, and mass cytometry. All these methods are covered in this short review.
Inductively coupled plasma mass spectrometry - single-cell analysis
Literatura
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- $a The cell, as a fundamental structural, functional and biological unit, plays an essential role in living organisms. Analysis of the molecular/elemental composition of a single cell is a significant aspect of lipidomic, proteomic, metabolomic and metallomic studies aiming at understanding molecular processes in cells. Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful technique which can help to elucidate the bioeffects of trace metals and their species on cellular metabolism and cell behavior. Numerous ICP-MS-based methods have already been utilized to explore elemental/species profiles. These include, e.g. time-resolved ICP-MS, electrothermal vaporization ICP-MS, laser ablation ICP-MS, chip-based microextraction techniques, HPLC/CE-ICP-MS, elemental tagging, and mass cytometry. All these methods are covered in this short review.
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