Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Inhalation of molecular hydrogen prevents ischemia-reperfusion liver damage during major liver resection

O. Malý, J. Zajak, R. Hyšpler, Z. Turek, D. Astapenko, D. Jun, N. Váňová, A. Kohout, V. Radochová, J. Kotek, J. Páral,

. 2019 ; 7 (23) : 774. [pub] -

Jazyk angličtina Země Čína

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc20005209

Background: Liver resection is a surgical procedure associated with a high risk of hepatic failure that can be fatal. One of the key mechanisms involves ischemia-reperfusion damage. Building on the well-known positive effects of hydrogen at mitigating this damage, the goal of this work was to demonstrate the antioxidant, anti-inflammatory, and anti-apoptotic effects of inhaled hydrogen in domestic pigs during major liver resection. Methods: The study used a total of 12 domestic pigs, 6 animals underwent resection with inhaled hydrogen during general anesthesia, and 6 animals underwent the same procedure using conventional, unsupplemented, general anesthesia. Intraoperative preparation of the left branch of the hepatic portal vein and the left hepatic artery was performed, and a tourniquet was applied. Warm ischemia was induced for 120 minutes and then followed by liver reperfusion for another 120 minutes. Samples from the ischemic and non-ischemic halves of the liver were then removed for histological and biochemical examinations. Results: An evaluation of histological changes was based on a numerical expression of damage based on the Suzuki score. Liver samples in the group with inhaled hydrogen showed a statistically significant reduction in histological changes compared to the control group. Biochemical test scores showed no statistically significant difference in hepatic transaminases, alkaline phosphatase (ALP), lactate dehydrogenase (LD), and lactate. However, a surprising result was a statistically significant difference in gamma-glutamyl-transferase (GMT). Marker levels of oxidative damage varied noticeably in plasma samples. Conclusions: In this experimental study, we showed that inhaled hydrogen during major liver resection unquestionably reduced the level of oxidative stress associated with ischemia-reperfusion damage. We confirmed this phenomenon both histologically and by direct measurement of oxidative stress in the organism.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20005209
003      
CZ-PrNML
005      
20230815105418.0
007      
ta
008      
200511s2019 cc f 000 0|eng||
009      
AR
024    7_
$a 10.21037/atm.2019.11.43 $2 doi
035    __
$a (PubMed)32042790
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a cc
100    1_
$a Malý, Ondřej $u Department of Military Surgery, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic. Department of Surgery, University Hospital Hradec Králové, Hradec Králové, Czech Republic.
245    10
$a Inhalation of molecular hydrogen prevents ischemia-reperfusion liver damage during major liver resection / $c O. Malý, J. Zajak, R. Hyšpler, Z. Turek, D. Astapenko, D. Jun, N. Váňová, A. Kohout, V. Radochová, J. Kotek, J. Páral,
520    9_
$a Background: Liver resection is a surgical procedure associated with a high risk of hepatic failure that can be fatal. One of the key mechanisms involves ischemia-reperfusion damage. Building on the well-known positive effects of hydrogen at mitigating this damage, the goal of this work was to demonstrate the antioxidant, anti-inflammatory, and anti-apoptotic effects of inhaled hydrogen in domestic pigs during major liver resection. Methods: The study used a total of 12 domestic pigs, 6 animals underwent resection with inhaled hydrogen during general anesthesia, and 6 animals underwent the same procedure using conventional, unsupplemented, general anesthesia. Intraoperative preparation of the left branch of the hepatic portal vein and the left hepatic artery was performed, and a tourniquet was applied. Warm ischemia was induced for 120 minutes and then followed by liver reperfusion for another 120 minutes. Samples from the ischemic and non-ischemic halves of the liver were then removed for histological and biochemical examinations. Results: An evaluation of histological changes was based on a numerical expression of damage based on the Suzuki score. Liver samples in the group with inhaled hydrogen showed a statistically significant reduction in histological changes compared to the control group. Biochemical test scores showed no statistically significant difference in hepatic transaminases, alkaline phosphatase (ALP), lactate dehydrogenase (LD), and lactate. However, a surprising result was a statistically significant difference in gamma-glutamyl-transferase (GMT). Marker levels of oxidative damage varied noticeably in plasma samples. Conclusions: In this experimental study, we showed that inhaled hydrogen during major liver resection unquestionably reduced the level of oxidative stress associated with ischemia-reperfusion damage. We confirmed this phenomenon both histologically and by direct measurement of oxidative stress in the organism.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Zajak, Ján $u Department of Surgery, University Hospital Hradec Králové, Hradec Králové, Czech Republic.
700    1_
$a Hyšpler, Radomír $u Institute of Clinical Biochemistry and Diagnostics, Resuscitation and Intensive Medicine, University Hospital Hradec Králové, Hradec Králové, Czech Republic. Center for Development and Research, Resuscitation and Intensive Medicine, University Hospital Hradec Králové, Hradec Králové, Czech Republic.
700    1_
$a Turek, Zdeněk $u Department of Anesthesiology, Resuscitation and Intensive Medicine, University Hospital Hradec Králové, Hradec Králové, Czech Republic.
700    1_
$a Astapenko, David $u Department of Anesthesiology, Resuscitation and Intensive Medicine, University Hospital Hradec Králové, Hradec Králové, Czech Republic.
700    1_
$a Jun, Daniel $u Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic.
700    1_
$a Váňová, Nela $u Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Hradec Králové, Czech Republic.
700    1_
$a Kohout, Aleš $u Fingerland Institute of Pathology, University Hospital Hradec Králové, Hradec Králové, Czech Republic.
700    1_
$a Radochová, Věra $u Department of Vivarium, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic.
700    1_
$a Kotek, Jiří $u Department of Military Surgery, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic. $7 xx0305380
700    1_
$a Páral, Jiří $u Department of Military Surgery, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic. Department of Surgery, University Hospital Hradec Králové, Hradec Králové, Czech Republic.
773    0_
$w MED00186147 $t Annals of translational medicine $x 2305-5839 $g Roč. 7, č. 23 (2019), s. 774
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32042790 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200511 $b ABA008
991    __
$a 20230815105415 $b ABA008
999    __
$a ind $b bmc $g 1524139 $s 1095264
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 7 $c 23 $d 774 $e - $i 2305-5839 $m Annals of translational medicine $n Ann Transl Med $x MED00186147
LZP    __
$a Pubmed-20200511

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace