Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica

D. Nováková, P. Švec, M. Zeman, HJ. Busse, I. Mašlaňová, R. Pantůček, S. Králová, L. Krištofová, I. Sedláček,

. 2020 ; 70 (1) : 302-308. [pub] -

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc20005794

Grantová podpora
NV16-29916A MZ0 CEP - Centrální evidence projektů

A taxonomic study was carried out on four Gram-stain-negative strains P5773T, P6169, P4708 and P6245, isolated from anus or mouth samples of Weddell seals at James Ross Island, Antarctica. The results of initial 16S rRNA gene sequence analysis showed that all four strains formed a group placed in the genus Pseudomonas and found Pseudomonas guineae and Pseudomonas peli to be their closest neighbours with 99.9 and 99.2 % sequence similarity, respectively. Sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of isolates to P. peli (rpoD) and to P. guineae (rpoB and gyrB). The average nucleotide identity value below 86 %, as calculated from the whole-genome sequence data, showed the low genomic relatedness of P5773T to its phylogenetic neighbours. The complete genome of strain P5773T was 4.4 Mb long and contained genes encoding proteins with biotechnological potential. The major fatty acids of the seal isolates were summed feature 8 (C18 : 1ω7c), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16:0. The major respiratory quinone was Q9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Putrescine and spermidine are predominant in the polyamine pattern. Further characterization performed using repetitive sequence-based PCR fingerprinting and MALDI-TOF MS analysis showed that the studied isolates formed a coherent cluster separated from the remaining Pseudomonas species and confirmed that they represent a novel species within the genus Pseudomonas, for which the name Pseudomonasleptonychotis sp. nov. is suggested. The type strain is P5773T (=CCM 8849T=LMG 30618T).

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20005794
003      
CZ-PrNML
005      
20200525085154.0
007      
ta
008      
200511s2020 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1099/ijsem.0.003753 $2 doi
035    __
$a (PubMed)31617844
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Nováková, Dana $u Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
245    10
$a Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica / $c D. Nováková, P. Švec, M. Zeman, HJ. Busse, I. Mašlaňová, R. Pantůček, S. Králová, L. Krištofová, I. Sedláček,
520    9_
$a A taxonomic study was carried out on four Gram-stain-negative strains P5773T, P6169, P4708 and P6245, isolated from anus or mouth samples of Weddell seals at James Ross Island, Antarctica. The results of initial 16S rRNA gene sequence analysis showed that all four strains formed a group placed in the genus Pseudomonas and found Pseudomonas guineae and Pseudomonas peli to be their closest neighbours with 99.9 and 99.2 % sequence similarity, respectively. Sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of isolates to P. peli (rpoD) and to P. guineae (rpoB and gyrB). The average nucleotide identity value below 86 %, as calculated from the whole-genome sequence data, showed the low genomic relatedness of P5773T to its phylogenetic neighbours. The complete genome of strain P5773T was 4.4 Mb long and contained genes encoding proteins with biotechnological potential. The major fatty acids of the seal isolates were summed feature 8 (C18 : 1ω7c), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16:0. The major respiratory quinone was Q9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Putrescine and spermidine are predominant in the polyamine pattern. Further characterization performed using repetitive sequence-based PCR fingerprinting and MALDI-TOF MS analysis showed that the studied isolates formed a coherent cluster separated from the remaining Pseudomonas species and confirmed that they represent a novel species within the genus Pseudomonas, for which the name Pseudomonasleptonychotis sp. nov. is suggested. The type strain is P5773T (=CCM 8849T=LMG 30618T).
650    _2
$a zvířata $7 D000818
650    _2
$a techniky typizace bakterií $7 D015373
650    _2
$a zastoupení bazí $7 D001482
650    _2
$a DNA bakterií $x genetika $7 D004269
650    _2
$a mastné kyseliny $x chemie $7 D005227
650    _2
$a bakteriální geny $7 D005798
650    _2
$a fosfolipidy $x chemie $7 D010743
650    12
$a fylogeneze $7 D010802
650    _2
$a Pseudomonas $x klasifikace $7 D011549
650    _2
$a RNA ribozomální 16S $x genetika $7 D012336
650    _2
$a tuleňovití $x mikrobiologie $7 D046048
650    _2
$a sekvenční analýza DNA $7 D017422
650    _2
$a ubichinon $x chemie $7 D014451
651    _2
$a Antarktida $7 D000864
655    _2
$a časopisecké články $7 D016428
700    1_
$a Švec, Pavel $u Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
700    1_
$a Zeman, Michal $u Section of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
700    1_
$a Busse, Hans-Jürgen $u Institute of Microbiology, University of Veterinary Medicine Vienna, A-1210 Vienna, Austria.
700    1_
$a Mašlaňová, Ivana $u Section of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
700    1_
$a Pantůček, Roman $u Section of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
700    1_
$a Králová, Stanislava $u Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
700    1_
$a Krištofová, Lucie $u Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
700    1_
$a Sedláček, Ivo $u Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
773    0_
$w MED00006002 $t International journal of systematic and evolutionary microbiology $x 1466-5034 $g Roč. 70, č. 1 (2020), s. 302-308
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31617844 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200511 $b ABA008
991    __
$a 20200525085154 $b ABA008
999    __
$a ok $b bmc $g 1524652 $s 1095850
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 70 $c 1 $d 302-308 $e - $i 1466-5034 $m International journal of systematic and evolutionary microbiology $n Int. j. syst. evol. microbiol. (Print) $x MED00006002
GRA    __
$a NV16-29916A $p MZ0
LZP    __
$a Pubmed-20200511

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...