-
Je něco špatně v tomto záznamu ?
Study of interactions between carboxylated core shell magnetic nanoparticles and polymyxin B by capillary electrophoresis with inductively coupled plasma mass spectrometry
D. Baron, J. Rozsypal, A. Michel, E. Secret, JM. Siaugue, T. Pluháček, J. Petr,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- elektroforéza kapilární metody MeSH
- hmotnostní spektrometrie metody MeSH
- magnetické nanočástice chemie MeSH
- polymyxin B analýza MeSH
- tlak MeSH
- Publikační typ
- časopisecké články MeSH
In this work, interactions of carboxylated core shell magnetic nanoparticles with polymyxin B sulfate were studied by connecting capillary electrophoresis with inductively coupled plasma mass spectrometry. The interaction was probed by affinity mode of capillary electrophoresis with 25 mM phosphate buffer at physiological pH. 54Fe, 56Fe, 57Fe, 34S, and 12C isotopes were used to monitor the migration of an electroosmotic flow marker and the interaction of the nanoparticles with polymyxin B. The analysis of interaction data showed two distinct interaction regions, one with low polymyxin B concentration, the second with high polymyxin B concentration. These regions differed in the strength of the interaction, 1.49 × 107 M-1 and 1.60 × 104 M-1, and in the stoichiometry of 0.7 and 3.5, respectively. These differences can be explained by the decrease of electrostatic repulsion between nanoparticles caused by polymyxin B. This is also in agreement with the nanoparticles peak shapes: sharp for low polymyxin B concentrations and broad for high polymyxin B concentrations.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20006022
- 003
- CZ-PrNML
- 005
- 20200518132251.0
- 007
- ta
- 008
- 200511s2020 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.chroma.2019.460433 $2 doi
- 035 __
- $a (PubMed)31427136
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Baron, Daniel $u Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic.
- 245 10
- $a Study of interactions between carboxylated core shell magnetic nanoparticles and polymyxin B by capillary electrophoresis with inductively coupled plasma mass spectrometry / $c D. Baron, J. Rozsypal, A. Michel, E. Secret, JM. Siaugue, T. Pluháček, J. Petr,
- 520 9_
- $a In this work, interactions of carboxylated core shell magnetic nanoparticles with polymyxin B sulfate were studied by connecting capillary electrophoresis with inductively coupled plasma mass spectrometry. The interaction was probed by affinity mode of capillary electrophoresis with 25 mM phosphate buffer at physiological pH. 54Fe, 56Fe, 57Fe, 34S, and 12C isotopes were used to monitor the migration of an electroosmotic flow marker and the interaction of the nanoparticles with polymyxin B. The analysis of interaction data showed two distinct interaction regions, one with low polymyxin B concentration, the second with high polymyxin B concentration. These regions differed in the strength of the interaction, 1.49 × 107 M-1 and 1.60 × 104 M-1, and in the stoichiometry of 0.7 and 3.5, respectively. These differences can be explained by the decrease of electrostatic repulsion between nanoparticles caused by polymyxin B. This is also in agreement with the nanoparticles peak shapes: sharp for low polymyxin B concentrations and broad for high polymyxin B concentrations.
- 650 _2
- $a elektroforéza kapilární $x metody $7 D019075
- 650 _2
- $a magnetické nanočástice $x chemie $7 D058185
- 650 _2
- $a hmotnostní spektrometrie $x metody $7 D013058
- 650 _2
- $a polymyxin B $x analýza $7 D011112
- 650 _2
- $a tlak $7 D011312
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Rozsypal, Jan $u Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic.
- 700 1_
- $a Michel, Aude $u Sorbonne Université, CNRS, Physico-chimie des Électrolytes et Nanosystèmes Interfaciaux, PHENIX, F-75005 Paris, France.
- 700 1_
- $a Secret, Emilie $u Sorbonne Université, CNRS, Physico-chimie des Électrolytes et Nanosystèmes Interfaciaux, PHENIX, F-75005 Paris, France.
- 700 1_
- $a Siaugue, Jean-Michel $u Sorbonne Université, CNRS, Physico-chimie des Électrolytes et Nanosystèmes Interfaciaux, PHENIX, F-75005 Paris, France.
- 700 1_
- $a Pluháček, Tomáš $u Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic. Electronic address: tomas.pluhacek@upol.cz.
- 700 1_
- $a Petr, Jan $u Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic. Electronic address: jan.petr@upol.cz.
- 773 0_
- $w MED00004962 $t Journal of chromatography. A $x 1873-3778 $g Roč. 1609, č. - (2020), s. 460433
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31427136 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20200511 $b ABA008
- 991 __
- $a 20200518132251 $b ABA008
- 999 __
- $a ok $b bmc $g 1524880 $s 1096078
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 1609 $c - $d 460433 $e 20190809 $i 1873-3778 $m Journal of chromatography. A, Including electrophoresis and other separation methods $n J Chromatogr A $x MED00004962
- LZP __
- $a Pubmed-20200511
