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Je něco špatně v tomto záznamu ?
The biochemistry underpinning industrial seed technology and mechanical processing of sugar beet
M. Ignatz, JE. Hourston, V. Turečková, M. Strnad, J. Meinhard, U. Fischer, T. Steinbrecher, G. Leubner-Metzger,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
Grantová podpora
direct funding from industry
KWS SAAT SE
BB/M000583/1
Biotechnology and Biological Sciences Research Council - United Kingdom
CZ.02.1.01/0.0/0.0/16_019/0000827
ERDF
NLK
ProQuest Central
od 2002-11-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 1999-11-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2002-11-01 do Před 1 rokem
- MeSH
- Beta vulgaris růst a vývoj fyziologie MeSH
- biochemie MeSH
- klíčení MeSH
- kyselina abscisová metabolismus MeSH
- regulátory růstu rostlin metabolismus MeSH
- semena rostlinná růst a vývoj fyziologie MeSH
- tandemová hmotnostní spektrometrie MeSH
- Publikační typ
- časopisecké články MeSH
MAIN CONCLUSION: Seed-processing technologies such as polishing and washing enhance crop seed quality by limited removal of the outer layers and by leaching. Combined, this removes chemical compounds that inhibit germination. Industrial processing to deliver high-quality commercial seed includes removing chemical inhibitors of germination, and is essential to produce fresh sprouts, achieve vigorous crop establishment, and high yield potential in the field. Sugar beet (Beta vulgaris subsp. vulgaris var. altissima Doell.), the main sugar source of the temperate agricultural zone, routinely undergoes several processing steps during seed production to improve germination performance and seedling growth. Germination assays and seedling phenotyping was carried out on unprocessed, and processed (polished and washed) sugar beet fruits. Pericarp-derived solutes, known to inhibit germination, were tested in germination assays and their osmolality and conductivity assessed (ions). Abscisic acid (ABA) and ABA metabolites were quantified in both the true seed and pericarp tissue using UPLC-ESI(+)-MS/MS. Physical changes in the pericarp structures were assessed using scanning electron microscopy (SEM). We found that polishing and washing of the sugar beet fruits both had a positive effect on germination performance and seedling phenotype, and when combined, this positive effect was stronger. The mechanical action of polishing removed the outer pericarp (fruit coat) tissue (parenchyma), leaving the inner tissue (sclerenchyma) unaltered, as revealed by SEM. Polishing as well as washing removed germination inhibitors from the pericarp, specifically, ABA, ABA metabolites, and ions. Understanding the biochemistry underpinning the effectiveness of these processing treatments is key to driving further innovations in commercial seed quality.
Department of Biological Sciences Royal Holloway University of London Egham Surrey TW20 0EX UK
KWS SAAT SE and Co KGaA Grimsehlstr 31 37555 Einbeck Germany
Citace poskytuje Crossref.org
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- $a MAIN CONCLUSION: Seed-processing technologies such as polishing and washing enhance crop seed quality by limited removal of the outer layers and by leaching. Combined, this removes chemical compounds that inhibit germination. Industrial processing to deliver high-quality commercial seed includes removing chemical inhibitors of germination, and is essential to produce fresh sprouts, achieve vigorous crop establishment, and high yield potential in the field. Sugar beet (Beta vulgaris subsp. vulgaris var. altissima Doell.), the main sugar source of the temperate agricultural zone, routinely undergoes several processing steps during seed production to improve germination performance and seedling growth. Germination assays and seedling phenotyping was carried out on unprocessed, and processed (polished and washed) sugar beet fruits. Pericarp-derived solutes, known to inhibit germination, were tested in germination assays and their osmolality and conductivity assessed (ions). Abscisic acid (ABA) and ABA metabolites were quantified in both the true seed and pericarp tissue using UPLC-ESI(+)-MS/MS. Physical changes in the pericarp structures were assessed using scanning electron microscopy (SEM). We found that polishing and washing of the sugar beet fruits both had a positive effect on germination performance and seedling phenotype, and when combined, this positive effect was stronger. The mechanical action of polishing removed the outer pericarp (fruit coat) tissue (parenchyma), leaving the inner tissue (sclerenchyma) unaltered, as revealed by SEM. Polishing as well as washing removed germination inhibitors from the pericarp, specifically, ABA, ABA metabolites, and ions. Understanding the biochemistry underpinning the effectiveness of these processing treatments is key to driving further innovations in commercial seed quality.
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