-
Something wrong with this record ?
In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin
K. Michalcova, S. Roychoudhury, M. Halenar, E. Tvrda, E. Kovacikova, J. Vasicek, P. Chrenek, S. Baldovska, L. Sanislo, V. Kren, A. Kolesarova,
Language English Country Great Britain
Document type Journal Article
- MeSH
- Antioxidants pharmacology MeSH
- Apoptosis drug effects MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Ovarian Neoplasms drug therapy genetics metabolism physiopathology MeSH
- Oxidative Stress drug effects MeSH
- Quercetin analogs & derivatives pharmacology MeSH
- Reactive Oxygen Species metabolism MeSH
- Signal Transduction drug effects MeSH
- Transforming Growth Factor beta1 genetics metabolism MeSH
- Cell Survival drug effects MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-β1 (TGF-β1) and TGF-β1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at concentrations 5, 10, 25, 50, and 100 μg mL-1. AlamarBlue assay revealed that isoquercitrin did not cause any significant change (P > 0.05) in cell viability as compared to control. Apoptotic assay using flow cytometry did not find any significant change (P > 0.05) in the proportion of live, dead and apoptotic cells as compared to control. ELISA also showed that the release of human TGF-β1 and TGF-β1 receptor were not significantly (P > 0.05) affected by isoquercitrin as compared to control. Chemiluminescence assay demonstrated that lower concentrations (5, 10, and 25 μg mL-1) were able to exhibit beneficial effects by inhibiting the generation of intracellular ROS. In contrast, elevated concentrations of 50 and 100 μg mL-1 led to oxidative stress (P < 0.05). We concluded that the beneficial effect of isoquercitrin on ovarian cancer cells may be mediated by an antioxidative pathway that involves inhibition of intracellular ROS generation, thereby limiting oxidative stress.
Institute of Microbiology Czech Academy of Sciences Prague 4 Czech Republic
Research Centre AgroBioTech Slovak University of Agriculture in Nitra Nitra Slovak Republic
St Elizabeth Cancer Institute Hospital Bratislava Slovak Republic
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20006193
- 003
- CZ-PrNML
- 005
- 20210917110437.0
- 007
- ta
- 008
- 200511s2019 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1080/03601234.2019.1633214 $2 doi
- 035 __
- $a (PubMed)31271108
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Michalcova, Katarina $u Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.
- 245 10
- $a In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin / $c K. Michalcova, S. Roychoudhury, M. Halenar, E. Tvrda, E. Kovacikova, J. Vasicek, P. Chrenek, S. Baldovska, L. Sanislo, V. Kren, A. Kolesarova,
- 520 9_
- $a Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-β1 (TGF-β1) and TGF-β1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at concentrations 5, 10, 25, 50, and 100 μg mL-1. AlamarBlue assay revealed that isoquercitrin did not cause any significant change (P > 0.05) in cell viability as compared to control. Apoptotic assay using flow cytometry did not find any significant change (P > 0.05) in the proportion of live, dead and apoptotic cells as compared to control. ELISA also showed that the release of human TGF-β1 and TGF-β1 receptor were not significantly (P > 0.05) affected by isoquercitrin as compared to control. Chemiluminescence assay demonstrated that lower concentrations (5, 10, and 25 μg mL-1) were able to exhibit beneficial effects by inhibiting the generation of intracellular ROS. In contrast, elevated concentrations of 50 and 100 μg mL-1 led to oxidative stress (P < 0.05). We concluded that the beneficial effect of isoquercitrin on ovarian cancer cells may be mediated by an antioxidative pathway that involves inhibition of intracellular ROS generation, thereby limiting oxidative stress.
- 650 _2
- $a antioxidancia $x farmakologie $7 D000975
- 650 _2
- $a apoptóza $x účinky léků $7 D017209
- 650 _2
- $a nádorové buněčné linie $7 D045744
- 650 _2
- $a viabilita buněk $x účinky léků $7 D002470
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a nádory vaječníků $x farmakoterapie $x genetika $x metabolismus $x patofyziologie $7 D010051
- 650 _2
- $a oxidační stres $x účinky léků $7 D018384
- 650 _2
- $a quercetin $x analogy a deriváty $x farmakologie $7 D011794
- 650 _2
- $a reaktivní formy kyslíku $x metabolismus $7 D017382
- 650 _2
- $a signální transdukce $x účinky léků $7 D015398
- 650 _2
- $a transformující růstový faktor beta1 $x genetika $x metabolismus $7 D053773
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Roychoudhury, Shubhadeep $u Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic. Department of Life Science and Bioinformatics , Assam University , Silchar , India.
- 700 1_
- $a Halenar, Marek $u Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.
- 700 1_
- $a Tvrdá, Eva $u Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic. $7 xx0264143
- 700 1_
- $a Kovacikova, Eva $u Research Centre AgroBioTech , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.
- 700 1_
- $a Vasicek, Jaromir $u Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic. Research Institute for Animal Production Nitra , National Agricultural and Food Centre , Lužianky , Slovak Republic.
- 700 1_
- $a Chrenek, Peter $u Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic. Research Institute for Animal Production Nitra , National Agricultural and Food Centre , Lužianky , Slovak Republic.
- 700 1_
- $a Baldovska, Simona $u Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.
- 700 1_
- $a Sanislo, Luboslav $u St. Elizabeth Cancer Institute Hospital , Bratislava , Slovak Republic.
- 700 1_
- $a Kren, Vladimir $u Institute of Microbiology , Czech Academy of Sciences , Prague 4 , Czech Republic.
- 700 1_
- $a Kolesárová, Adriana, $d 1975- $u Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic. $7 xx0238611
- 773 0_
- $w MED00007281 $t Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes $x 1532-4109 $g Roč. 54, č. 9 (2019), s. 752-757
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31271108 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20200511 $b ABA008
- 991 __
- $a 20210917110435 $b ABA008
- 999 __
- $a ok $b bmc $g 1525051 $s 1096249
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 54 $c 9 $d 752-757 $e 20190704 $i 1532-4109 $m Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes $n J Environ Sci Health B $x MED00007281
- LZP __
- $a Pubmed-20200511