-
Je něco špatně v tomto záznamu ?
Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes
M. Potocký, R. Bezvoda, P. Pejchar,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antisense oligodeoxyribonukleotidy genetika MeSH
- genový knockdown metody MeSH
- pylová láčka genetika MeSH
- regulace genové exprese u rostlin MeSH
- tabák genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20006308
- 003
- CZ-PrNML
- 005
- 20200518132530.0
- 007
- ta
- 008
- 200511s2019 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-4939-9469-4_24 $2 doi
- 035 __
- $a (PubMed)31148051
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Potocký, Martin $u Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic. potocky@ueb.cas.cz. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic. potocky@ueb.cas.cz.
- 245 10
- $a Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes / $c M. Potocký, R. Bezvoda, P. Pejchar,
- 520 9_
- $a Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
- 650 _2
- $a regulace genové exprese u rostlin $7 D018506
- 650 _2
- $a genový knockdown $x metody $7 D055785
- 650 _2
- $a antisense oligodeoxyribonukleotidy $x genetika $7 D020319
- 650 _2
- $a pylová láčka $x genetika $7 D053205
- 650 _2
- $a tabák $x genetika $7 D014026
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Bezvoda, Radek $u Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.
- 700 1_
- $a Pejchar, Přemysl $u Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.
- 773 0_
- $w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 1992, č. - (2019), s. 359-365
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31148051 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20200511 $b ABA008
- 991 __
- $a 20200518132530 $b ABA008
- 999 __
- $a ok $b bmc $g 1525166 $s 1096364
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 1992 $c - $d 359-365 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20200511
