Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine

V. Patsula, J. Tulinska, Š. Trachtová, M. Kuricova, A. Liskova, A. Španová, F. Ciampor, I. Vavra, B. Rittich, M. Ursinyova, M. Dusinska, S. Ilavska, M. Horvathova, V. Masanova, I. Uhnakova, D. Horák,

. 2019 ; 13 (4) : 510-526. [pub] 20190201

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20006614

Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12 nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles; conventional γ-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25 pg Fe/cell for HA-PEG@Fe3O4, γ-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12-75 μg/cm2) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of γ-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 μg/cm2, 72 h) and also decreased the phagocytic activity of monocytes (15 μg/cm2, 24 h; 3-75 μg/cm2, 72 h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20006614
003      
CZ-PrNML
005      
20201014161816.0
007      
ta
008      
200511s2019 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1080/17435390.2018.1555624 $2 doi
035    __
$a (PubMed)30704361
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Patsula, Vitalii, $d 1988- $7 xx0252776 $u Institute of Macromolecular Chemistry, Czech Academy of Sciences , Prague , Czech Republic.
245    10
$a Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine / $c V. Patsula, J. Tulinska, Š. Trachtová, M. Kuricova, A. Liskova, A. Španová, F. Ciampor, I. Vavra, B. Rittich, M. Ursinyova, M. Dusinska, S. Ilavska, M. Horvathova, V. Masanova, I. Uhnakova, D. Horák,
520    9_
$a Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12 nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles; conventional γ-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25 pg Fe/cell for HA-PEG@Fe3O4, γ-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12-75 μg/cm2) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of γ-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 μg/cm2, 72 h) and also decreased the phagocytic activity of monocytes (15 μg/cm2, 24 h; 3-75 μg/cm2, 72 h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy.
650    _2
$a proliferace buněk $x účinky léků $7 D049109
650    _2
$a viabilita buněk $x účinky léků $x imunologie $7 D002470
650    _2
$a kultivované buňky $7 D002478
650    _2
$a cytokiny $x metabolismus $7 D016207
650    _2
$a lidé $7 D006801
650    _2
$a kyseliny hydroxamové $x chemie $7 D006877
650    _2
$a leukocyty mononukleární $x účinky léků $x imunologie $x patologie $7 D007963
650    _2
$a magnetické nanočástice $x chemie $x toxicita $7 D058185
650    _2
$a nanomedicína $x metody $7 D050997
650    _2
$a velikost částic $7 D010316
650    _2
$a fagocytóza $x účinky léků $x imunologie $7 D010587
650    _2
$a kyseliny fosforité $x chemie $7 D010757
650    _2
$a polyethylenglykoly $x chemie $7 D011092
650    _2
$a respirační vzplanutí $x účinky léků $x imunologie $7 D016897
650    _2
$a povrchové vlastnosti $7 D013499
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Tulinska, Jana $u b Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
700    1_
$a Trachtová, Štěpánka $u c Institute of Food Science and Biotechnology, Brno University of Technology , Brno , Czech Republic.
700    1_
$a Kuricova, Miroslava $u b Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
700    1_
$a Liskova, Aurelia $u b Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
700    1_
$a Španová, Alena $u c Institute of Food Science and Biotechnology, Brno University of Technology , Brno , Czech Republic.
700    1_
$a Ciampor, Fedor $u d Institute of Virology, Slovak Academy of Sciences , Bratislava , Slovakia.
700    1_
$a Vavra, Ivo $u e Institute of Electrical Engineering, Slovak Academy of Sciences , Bratislava , Slovakia.
700    1_
$a Rittich, Bohuslav $u c Institute of Food Science and Biotechnology, Brno University of Technology , Brno , Czech Republic.
700    1_
$a Ursinyova, Monika $u b Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
700    1_
$a Dusinska, Mária $u f Health Effects Laboratory, Department of Environmental Chemistry , NILU-Norwegian Institute for Air Research , Jeller , Norway.
700    1_
$a Ilavska, Silvia $u b Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
700    1_
$a Horvathova, Mira $u b Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
700    1_
$a Masanova, Vlasta $u b Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
700    1_
$a Uhnakova, Iveta $u b Faculty of Medicine , Slovak Medical University , Bratislava , Slovakia.
700    1_
$a Horák, Daniel $u a Institute of Macromolecular Chemistry, Czech Academy of Sciences , Prague , Czech Republic.
773    0_
$w MED00192827 $t Nanotoxicology $x 1743-5404 $g Roč. 13, č. 4 (2019), s. 510-526
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30704361 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200511 $b ABA008
991    __
$a 20201014161747 $b ABA008
999    __
$a ok $b bmc $g 1525472 $s 1096670
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 13 $c 4 $d 510-526 $e 20190201 $i 1743-5404 $m Nanotoxicology $n Nanotoxicology $x MED00192827
LZP    __
$a Pubmed-20200511

Najít záznam

Citační ukazatele

Možnosti archivace