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Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling

IV. Khalo, AI. Konokhova, DY. Orlova, KV. Trusov, MA. Yurkin, E. Bartova, S. Kozubek, VP. Maltsev, AV. Chernyshev,

. 2018 ; 454 (-) : 60-69. [pub] 20180530

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20006820

The dynamics of nuclear morphology changes during apoptosis remains poorly investigated and understood. Using 3D time-lapse confocal microscopy we performed a study of early-stage apoptotic nuclear morphological changes induced by etoposide in single living HepG2 cells. These observations provide a definitive evidence that nuclear apoptotic volume decrease (AVD) is occurring simultaneously with peripheral chromatin condensation (so called "apoptotic ring"). In order to describe quantitatively the dynamics of nuclear morphological changes in the early stage of apoptosis we suggest a general molecular kinetic model, which fits well the obtained experimental data in our study. Results of this work may clarify molecular mechanisms of nuclear morphology changes during apoptosis.

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$a The dynamics of nuclear morphology changes during apoptosis remains poorly investigated and understood. Using 3D time-lapse confocal microscopy we performed a study of early-stage apoptotic nuclear morphological changes induced by etoposide in single living HepG2 cells. These observations provide a definitive evidence that nuclear apoptotic volume decrease (AVD) is occurring simultaneously with peripheral chromatin condensation (so called "apoptotic ring"). In order to describe quantitatively the dynamics of nuclear morphological changes in the early stage of apoptosis we suggest a general molecular kinetic model, which fits well the obtained experimental data in our study. Results of this work may clarify molecular mechanisms of nuclear morphology changes during apoptosis.
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$a Konokhova, Anastasiya I $u Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, Novosibirsk 630090, Russia.
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$a Orlova, Darya Y $u Department of Genetics, Stanford University, Campus Drive 279, Stanford, CA 94305, USA.
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$a Trusov, Konstantin V $u Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia.
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$a Yurkin, Maxim A $u Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia.
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$a Bartova, Eva $u Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, Brno CZ-612 65, Czech Republic.
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$a Kozubek, Stanislav $u Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, Brno CZ-612 65, Czech Republic.
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$a Maltsev, Valeri P $u Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia; Novosibirsk State Medical University, Krasny Prospect 52, Novosibirsk 630091, Russia.
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$a Chernyshev, Andrei V $u Voevodsky Institute of Chemical Kinetics and Combustion, Institutskaya 3, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia. Electronic address: chern@kinetics.nsc.ru.
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