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Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
K. Šimek, D. Sirova,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't, Video-Audio Media
PubMed
31566612
DOI
10.3791/59903
Knihovny.cz E-resources
- MeSH
- Bacteria metabolism MeSH
- Eukaryota MeSH
- Water Microbiology * MeSH
- Microbiota * MeSH
- Food Chain * MeSH
- Predatory Behavior * MeSH
- Rivers MeSH
- Tetrahymena physiology MeSH
- Carbon metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Video-Audio Media MeSH
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
Elucidating trophic interactions, such as predation and its effects, is a frequent task for many researchers in ecology. The study of microbial communities has many limitations, and determining a predator, prey, and predatory rates is often difficult. Presented here is an optimized method based on the addition of fluorescently labelled prey as a tracer, which allows for reliable quantitation of the grazing rates in aquatic predatory eukaryotes and estimation of nutrient transfer to higher trophic levels.
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